Anti-AKT1/2 Antibody | Boster Immunoleader PA1301 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-AKT1/2 Antibody

catalog :

PA1301

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot

publications citing this reagent :

Wan J, Che Y, Kang N, Wu W. SOCS3 blocks HIF-1α expression to inhibit proliferation and angiogenesis of human small cell lung cancer by downregulating activation of Akt, but not STAT3. Mol Med Rep. 2015;12:83-92 pubmed publisher
Xu K, Ding Q, Fang Z, Zheng J, Gao P, Lu Y, et al. Silencing of HIF-1alpha suppresses tumorigenicity of renal cell carcinoma through induction of apoptosis. Cancer Gene Ther. 2010;17:212-22 pubmed publisher

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Boster Immunoleader product webpage

product information

Product Name :

Anti-AKT1/2 Antibody

Catalog Number :

PA1301

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for RAC-alpha serine/threonine-protein kinase(AKT1) detection. Tested with WB in Human.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human

Applications :

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the N-terminus of human AKT1/2(70-90aa NTFIIRCLQWTTVIERTFHVE), identical to the related rat and mouse sequences.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Background :

AKT protein family, which members are also called protein kinases B(PKB) plays an important role in mammalian cellular signaling. In humans, there are three genes in the "Akt family": Akt1, Akt2, and Akt3. These genes code for enzymes that are members of the serine/threonine-specific protein kinase family. Akt1 is involved in cellular survival pathways, by inhibiting apoptotic processes. Akt1 is also able to induce protein synthesis pathways, and is therefore a key signaling protein in the cellular pathways that lead to skeletal muscle hypertrophy, and general tissue growth. Since it can block apoptosis, and thereby promote cell survival, Akt1 has been implicated as a major factor in many types of cancer. Akt(now also called Akt1) was originally identified as the oncogene in the transforming retrovirus, AKT8. AKT8 was isolated by Stephen Staal in the laboratory of Wallace P. Rowe; he subsequently cloned v-akt and human AKT1 and AKT2 while on staff at the Johns Hopkins Oncology Center. Akt2 is an important signaling molecule in the Insulin signaling pathway, it is required to induce glucose transport. Franke et al.(1995) show that AKT1 and AKT2 are activated by PDGF. The activation was rapid and specific, and it was abrogated by mutations in the Akt Pleckstrin homology(PH) domain. They identify that Akt is a novel target of PI 3-kinase and suggest that the Akt PH domain may be a mediator of PI 3-kinase signaling.

Reference :

1. Staal SP, Hartley JW, Rowe WP (July 1977). "Isolation of transforming murine leukemia viruses from mice with a high incidence of spontaneous lymphoma". Proc. Natl. Acad. Sci. U.S.A. 74 (7): 3065–7. 2. Staal SP (July 1987). "Molecular cloning of the akt oncogene and its human homologues AKT1 and AKT2: amplification of AKT1 in a primary human gastric adenocarcinoma". Proc. Natl. Acad. Sci. U.S.A. 84 (14): 5034–7. 3. Franke, T. F.; Yang, S.-I.; Chan, T. O.; Datta, K.; Kaziauskas, A.; Morrison, D. K.; Kaplan, D. R.; Tsichlis, P. N. : The protein kinase encoded by the Akt proto-oncogene is a target of the PDGF-activated phosphatidylinositol 3-kinase. Cell 81: 727-736, 1995

Gene Name :

AKT1

Protein Name :

RAC-alpha serine/threonine-protein kinase

Gene Full Name :

v-akt murine thymoma viral oncogene homolog 1

Synonyms :

AKT 1 antibody AKT 2 antibody AKT antibody AKT1 antibody AKT1 kinase antibody AKT2 antibody AKT2 kinase antibody C AKT antibody MGC99656 antibody Murine thymoma viral(v akt) homolog 2 antibody Oncogene AKT1 antibody PKB alpha antibody PKB antibody PKB beta antibody PKBB antibody PKBBETA antibody PRKBA antibody PRKBB antibody Protein kinase Akt 2 antibody Protein Kinase B Alpha antibody Protein kinase B antibody Protein kinase B beta antibody RAC antibody RAC alpha antibody RAC alpha serine/threonine protein kinase antibody RAC BETA antibody RAC beta serine threonine protein kinase antibody RAC PK alpha antibody RAC PK beta antibody Rac protein kinase alpha antibody Rac protein kinase beta antibody RACbeta antibody v akt murine thymoma viral oncogene homolog 1 antibody v akt murine thymoma viral oncogene homolog 2 antibody

Uniprot ID :

P31749

more info or order :

Boster Immunoleader product webpage