Anti-Cyclin D1 Antibody | Boster Immunoleader PA1245-1 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Cyclin D1 Antibody

catalog :

PA1245-1

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Cyclin D1 Antibody

Catalog Number :

PA1245-1

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for G1/S-specific cyclin-D1(CCND1) detection. Tested with WB in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, rat Predicted to work with: mouse

Applications :

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of mouse Cyclin D1(219-238aa SPNNFLSCYRTTHFLSRVIK), identical to the related rat sequence, and different from the related human sequence by three amino acids.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Background :

Cyclin D1, also known as CCND1, is a human gene. The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclin D1 encodes the regulatory subunit of a holoenzyme that phosphorylates and inactivates the retinoblastoma protein and promotes progression through the G1-S phase of the cell cycle. Amplification or overexpression of cyclin D1 plays pivotal roles in the development of a subset of human cancers including parathyroid adenoma, breast cancer, colon cancer, lymphoma, melanoma, and prostate cancer. The cyclin D1 gene is overexpressed in human breast cancers and is required for oncogene-induced tumorigenesis. Brisken et al.(2003) found that prolactin(PRL; 176760) induced IGF2(147470) mRNA and IGF2 induced cyclin D1 protein expression in mouse mammary epithelial cultures. And they also concluded that IGF2 is a mediator of prolactin-induced alveologenesis and that prolactin, IGF2, and cyclin D1 are components of a developmental pathway in mammary gland.

Reference :

1. Fu, M.; Wang, C.; Li, Z.; Sakamaki, T.; Pestell, R. G. : Minireview: Cyclin D1: normal and abnormal functions. Endocrinology 145: 5439-5447, 2004. 2. Wang, C.; Pattabiraman, N.; Zhou, J. N.; Fu, M.; Sakamaki, T.; Albanese, C.; Li, Z.; Wu, K.; Hulit, J.; Neumeister, P.; Novikoff, P. M.; Brownlee, M.; Scherer, P. E.; Jones, J. G.; Whitney, K. D.; Donehower, L. A.; Harris, E. L.; Rohan, T.; Johns, D. C.; Pestell, R. G. : Cyclin D1 repression of peroxisome proliferator-activated receptor gamma expression and transactivation. Molec. Cell. Biol. 23: 6159-6173, 2003. 3. Brisken, C.; Ayyannan, A.; Nguyen, C.; Heineman, A.; Reinhardt, F.; Tan, J.; Dey, S. K.; Dotto, G. P.; Weinberg, R. A. : IGF-2 is a mediator of prolactin-induced morphogenesis in the breast. Dev. Cell 3: 877-887, 2002. Note: Erratum: Dev. Cell 4: 283 only, 2003.

Gene Name :

CCND1

Protein Name :

G1/S-specific cyclin-D1

Gene Full Name :

cyclin D1

Synonyms :

AI327039 antibody B cell ccl/lymphoma 1 antibody B cell CLL/lymphoma 1 antibody B cell leukemia 1 antibody B-cell leukemia 1 antibody B cell lymphoma 1 protein antibody B-cell lymphoma 1 protein antibody BCL 1 antibody BCL-1 antibody BCL-1 oncogene antibody BCL1 antibody BCL1 oncogene antibody CCND 1 antibody CCND1 antibody CCND1 protein antibody CCND1/FSTL3 fusion gene, included antibody CCND1/IGHG1 fusion gene antibody CCND1/IGHG1 fusion gene, included antibody CCND1/IGLC1 fusion gene, included antibody CCND1/PTH fusion gene, included antibody CCND1_HUMAN antibody cD1 antibody Cyl 1 antibody D11S287E antibody G1/S specific cyclin D1 antibody G1/S-specific cyclin-D1 antibody Parathyroid adenomatosis 1 antibody PRAD1 antibody PRAD1 oncogene antibody U21B31 antibody

Uniprot ID :

P25322

more info or order :

Boster Immunoleader product webpage