Anti-Cytokeratin 8 Antibody | Boster Immunoleader PA1240 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Cytokeratin 8 Antibody

catalog :

PA1240

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

publications citing this reagent :

Luo J, Liang M, Yang X, Xu H, Shi D, Lu S. Establishment and biological characteristics comparison of Chinese swamp buffalo (Bubalus bubalis) fibroblast cell lines. In Vitro Cell Dev Biol Anim. 2014;50:7-15 pubmed publisher
Qi X, Qu Y, Nan Z, Jin Y, Zhao X, Wang A. Caprine endometrial stromal cells modulate the effects of steroid hormones on cytokine secretion by endometrial epithelial cells in vitro. Reprod Biol. 2012;12:309-15 pubmed publisher

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product information

Product Name :

Anti-Cytokeratin 8 Antibody

Catalog Number :

PA1240

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Keratin, type II cytoskeletal 8(KRT8) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human Predicted to work with: mouse, rat

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human; Predicted Species: Mouse, Rat

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human; Predicted Species: Mouse, Rat

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the N-terminus of human Cytokeratin 8(127-145aa QQQKTARSNMDNMFESYIN), different from the related rat and mouse sequences by one amino acid.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

Cytokeratin 8(CK8), also known as Keratin 8(K8), is a type II keratin. Among the various intermediate filament(IF) proteins, cytokeratin 8(CK8) is especially remarkable as it is produced early in embryogenesis, is the only type-II CK occurring in many simple epithelial cells, and can also be synthesized in certain non-epithelial cells. Cytokeratin 8 mRNA was abundant in fetal small intestine, placenta, pancreas, lung, liver, and kidney. Levels of cytokeratin 8 mRNA in placenta increased slightly during pregnancy. He et al.(2002) presented evidence that K8 is a new cytoplasmic target for JNK in Fas receptor-mediated signaling. The functional significance of this phosphorylation could relate to regulation of JNK signaling and/or regulation of keratin dynamics.

Reference :

1. Krauss, S.; Franke, W. W. : Organization and sequence of the human gene encoding cytokeratin 8. Gene 86: 241-249, 1990. 2. Yamamoto, R.; Kao, L.-C.; McKnight, C. E.; Strauss, J. F., III : Cloning and sequence of cDNA for human placental cytokeratin 8: regulation of the mRNA in trophoblastic cells by cAMP. Molec. Endocr. 4: 370-374, 1990. 3. He, T.; Stepulak, A.; Holmstrom, T. H.; Omary, M. B.; Eriksson, J. E. : The intermediate filament protein kinase 8 is a novel cytoplasmic substrate for c-Jun N-terminal kinase. J. Biol. Chem. 277: 10767-10774, 2002.

Gene Name :

KRT8

Protein Name :

Keratin, type II cytoskeletal 8

Gene Full Name :

keratin 8

Synonyms :

card2 antibody Cardiac autoantigen 2 120kD antibody CK 8 antibody CK-8 antibody ck8 antibody Cyk 8 antibody cyk8 antibody CYKER antibody Cytokeratin 8 antibody Cytokeratin endo A antibody Cytokeratin-8 antibody Cytokeratin8 antibody DreK8 antibody EndoA antibody k0 antibody k2c8 antibody K2C8_HUMAN antibody k8 antibody Keratin 8 antibody Keratin type II cytoskeletal 8 antibody Keratin, type II cytoskeletal 8 antibody Keratin-8 antibody Keratin8 antibody KO antibody Krt 2.8 antibody Krt 8 antibody krt8 antibody MGC118110 antibody MGC174782 antibody MGC53564 antibody MGC85764 antibody sb:cb186 antibody Type-II keratin Kb8 antibody

Uniprot ID :

P05787

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