Anti-XAF1 Antibody | Boster Immunoleader PA1218 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-XAF1 Antibody

catalog :

PA1218

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human

application :

western blot, immunohistochemistry - paraffin section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-XAF1 Antibody

Catalog Number :

PA1218

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for XIAP-associated factor 1(XAF1) detection. Tested with WB, IHC-P in Human.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human XAF1(283-301aa QEKCRWLASSKGKQVRNFS).

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

XIAP associated factor-1, also known as XAF1, is a human gene. X-linked inhibitor of apoptosis(XIAP; MIM 300079) is a potent member of the IAP family. All members of this family possess baculoviral IAP(BIR) repeats, cysteine-rich domains of approximately 80 amino acids that bind and inhibit caspases. XAF1 antagonizes the anticaspase activity of XIAP and may be important in mediating apoptosis resistance in cancer cells. And alteration in XAF1 and XIAP RNA expression levels may lead to increased apoptotic resistance and proliferation due to unregulated XIAP function in cancer cells.

Reference :

1. Fong, W. G.; Liston, P.; Rajcan-Separovic, E.; St. Jean, M.; Craig, C.; Korneluk, R. G. : Expression and genetic analysis of XIAP-associated factor 1 (XAF1) in cancer cell lines. Genomics 70: 113-122, 2000. 2. Liston, P.; Fong, W. G.; Kelly, N. L.; Toji, S.; Miyazaki, T.; Conte, D.; Tamai, K.; Craig, C. G.; McBurney, M. W.; Korneluk, R. G. : Identification of XAF1 as an antagonist of XIAP anti-caspase activity. Nature Cell Biol. 3: 128-133, 2001.

Gene Name :

XAF1

Protein Name :

XIAP-associated factor 1

Gene Full Name :

XIAP associated factor 1

Synonyms :

BIRC 4 binding protein antibody BIRC4 binding protein antibody BIRC4-binding protein antibody BIRC4BP antibody HSXIAPAF 1 antibody HSXIAPAF1 antibody XAF 1 antibody XAF1 antibody XAF1_HUMAN antibody XIAP associated factor 1 antibody XIAP-associated factor 1 antibody XIAPAF1 antibody

Uniprot ID :

Q6GPH4

more info or order :

Boster Immunoleader product webpage