Anti-PROM1 Antibody | Boster Immunoleader PA1217 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-PROM1 Antibody

catalog :

PA1217

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

application :

western blot, immunohistochemistry - paraffin section

publications citing this reagent :

Li X, Zhao Y, Sun M, Shi J, Ju R, Zhang C, et al. Multifunctional liposomes loaded with paclitaxel and artemether for treatment of invasive brain glioma. Biomaterials. 2014;35:5591-604 pubmed publisher
Terzuoli E, Meini S, Cucchi P, Catalani C, Cialdai C, Maggi C, et al. Antagonism of bradykinin B2 receptor prevents inflammatory responses in human endothelial cells by quenching the NF-kB pathway activation. PLoS ONE. 2014;9:e84358 pubmed publisher
Zhan K, Bai L, Xu J. Role of vascular endothelial progenitor cells in construction of new vascular loop. Microvasc Res. 2013;90:1-11 pubmed publisher
Zhang J, Lu G. Effect of 17β‑estradiol in rat bone marrow‑derived endothelial progenitor cells. Mol Med Rep. 2013;8:178-82 pubmed publisher

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-PROM1 Antibody

Catalog Number :

PA1217

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Prominin-1(PROM1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, rat Predicted to work with: mouse

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the N-terminus of human PROM1(171-191aa NHQVRTRIKRSRKLADSNFKD).

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

CD133, is a glycoprotein also known in humans and rodents as Prominin 1(PROM1). It is the founding member of pentaspan transmembrane glycoproteins(5-transmembrane, 5-TM), which specifically localizes to cellular protrusions. The gene of CD133 is located in 4p15.3. And most of the CD133 gene is contained in 23 exons distributed over more than 50 kb of genomic sequence. CD133 is expressed in hematopoietic stem cells, endothelial progenitor cells, glioblastomas, neuronal and glial stem cells and some other cell types.

Reference :

1. Corbeil D, Fargeas C, Huttner W (2001). "Rat prominin, like its mouse and human orthologues, is a pentaspan membrane glycoprotein". Biochem Biophys Res Commun 285 (4): 939–44. 2. Maw, M. A.; Corbeil, D.; Koch, J.; Hellwig, A.; Wilson-Wheeler, J. C.; Bridges, R. J.; Kumaramanickavel, G.; John, S.; Nancarrow, D.; Roper, K.; Weigmann, A.; Huttner, W. B.; Denton, M. J. : A frameshift mutation in prominin (mouse)-like 1 causes human retinal degeneration. Hum. Molec. Genet. 9: 27-34, 2000. 3. Corbeil D, Röper K, Hellwig A, Tavian M, Miraglia S, Watt S, Simmons P, Peault B, Buck D, Huttner W (2000). "The human AC133 hematopoietic stem cell antigen is also expressed in epithelial cells and targeted to plasma membrane protrusions". J Biol Chem 275 (8): 5512–20. Shmelkov S, St Clair R, Lyden D, Rafii S (2005). "AC133/CD133/Prominin-1". Int J Biochem Cell Biol 37 (4): 715–9.

Gene Name :

PROM1

Protein Name :

Prominin-1

Gene Full Name :

prominin 1

Synonyms :

AC133 Antigen AC133 CD133 antibody CORD12 hematopoietic stem cell antigen hProminin MCDR2 MSTP061 antibody PROM1 PROM 1 PROM-1 Prominin-1 Prominin 1 Prominin1 Prominin-like protein 1 PROML1 antibody RP41 STGD4

Uniprot ID :

O43490

more info or order :

Boster Immunoleader product webpage