Anti-Hsc70 Antibody | Boster Immunoleader PA1214 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Hsc70 Antibody

catalog :

PA1214

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

application :

western blot, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Hsc70 Antibody

Catalog Number :

PA1214

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Heat shock cognate 71 kDa protein(HSPA8) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, mouse, rat

Applications :

WB,IHC-P,IHC-F,ICC

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Mouse, Rat

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human, Mouse, Rat

Immunohistochemistry(Frozen Section) :

Concentration: 0.5-1μg/ml; Tested Species: Mouse, Rat; Predicted Species: Human

Immunocytochemistry :

Concentration: 0.5-1μg/ml; Tested Species: Human; Predicted Species: Mouse, Rat

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the N-terminus of human Hsc70(13-31aa TTYSCVGVFQHGKVEIIAN), identical to the related rat and mouse sequence.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P), IHC(F) and ICC.

Background :

The 70 kilodalton heat shock proteins(Hsp70s) are a family of ubiquitously expressed heat shock proteins. The Hsp70s are an important part of the cell's machinery for protein folding, and help to protect cells from stress. All of the Hsp70 proteins have three major functional domains: An N-terminal ATPase domain binds ATP(Adenosine triphosphate) and hydrolyzes it to ADP(Adenosine diphosphate); A substrate binding domain contains a groove with an affinity for neutral, hydrophobic amino acid residues; A C-terminal domain rich in alpha helical structure acts as a 'lid' for the substrate binding domain. By binding tightly to partially-synthesized peptide sequences(incomplete proteins), Hsp70 prevents them from aggregating and being rendered nonfunctional. And it also can act to protect cells from thermal or oxidative stress. Finally, Hsp70 seems to be able to participate in disposal of damaged or defective proteins. Interaction with CHIP(Carboxyl-terminus of Hsp70 Interacting Protein)–an E3 ubiquitin ligase–allows Hsp70 to pass proteins to the cell's ubiquitination and proteolysis pathways.

Reference :

1. Tavaria M, Gabriele T, Kola I, Anderson RL (April 1996). "A hitchhiker's guide to the human Hsp70 family". Cell Stress Chaperones 1 (1): 23–8. 2. Morano KA (October 2007). "New tricks for an old dog: the evolving world of Hsp70". Ann. N. Y. Acad. Sci. 1113: 1–14. 3. Luders, J.; Demand, J.; Hohfeld, J. (2000), Journal of Biological Chemistry 275 (7): 4613–461, http://www.jbc.org/cgi/content/full/275/7/4613, retrieved on 2009-04-07

Gene Name :

HSPA8

Protein Name :

Heat shock cognate 71 kDa protein

Gene Full Name :

heat shock 70kDa protein 8

Synonyms :

2410008N15Rik antibody Constitutive heat shock protein 70 antibody Heat shock 70 kDa protein 8 antibody Heat shock 70kD protein 10 antibody Heat shock 70kD protein 8 antibody Heat shock 70kDa protein 8 antibody Heat shock cognate 71 kDa protein antibody Heat shock cognate 71 kDa protein. antibody Heat shock cognate protein 54 antibody Heat shock cognate protein 71 kDa antibody Heat shock cognate protein 71-kDa antibody Heat shock protein 8 antibody Heat shock protein A8 antibody Heat-shock70-KD protein 10, formerly antibody HSC54 antibody HSC71 antibody Hsc73 antibody HSP71 antibody HSP73 antibody HSP7C_HUMAN antibody HSPA10 antibody HSPA8 antibody LAP1 antibody Lipopolysaccharide associated protein 1 antibody LPS associated protein 1 antibody LPS associated protein antibody MGC102007 antibody MGC106514 antibody MGC114311 antibody MGC118485 antibody MGC131511 antibody MGC29929 antibody N-myristoyltransferase inhibitor protein 71 antibody NIP71 antibody

Uniprot ID :

E9PSH5

more info or order :

Boster Immunoleader product webpage