Anti-COX2/Cyclooxygenase 2 Antibody | Boster Immunoleader PA1211 product information

product summary

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company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-COX2/Cyclooxygenase 2 Antibody

catalog :

PA1211

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

publications citing this reagent :

Cui L, Feng L, Zhang Z, Jia X. The anti-inflammation effect of baicalin on experimental colitis through inhibiting TLR4/NF-κB pathway activation. Int Immunopharmacol. 2014;23:294-303 pubmed publisher
Cao D, Zhang Z, Xie R, Jiang B, Ji R, Gao Y. Chemokine CXCL1 enhances inflammatory pain and increases NMDA receptor activity and COX-2 expression in spinal cord neurons via activation of CXCR2. Exp Neurol. 2014;261:328-36 pubmed publisher
Du B, Zhang Z, Li N. Madecassoside prevents Aβ(25-35)-induced inflammatory responses and autophagy in neuronal cells through the class III PI3K/Beclin-1/Bcl-2 pathway. Int Immunopharmacol. 2014;20:221-8 pubmed publisher
Ni Y, Jiang T, Cheng Q, Gu Z, Zhu Y, Zhang Z, et al. Protective effect of magnolol on lipopolysaccharide-induced acute lung injury in mice. Inflammation. 2012;35:1860-6 pubmed publisher
Zhong B, Shen H, Sun X, Wang H, Zhang Y, Sun Z. Additive effects of ulinastatin and docetaxel on growth of breast cancer xenograft in nude mice and expression of PGE2, IL-10, and IL-2 in primary breast cancer cells. Cancer Biother Radiopharm. 2012;27:252-8 pubmed publisher
Ni Y, Kuai J, Lu Z, Yang G, Fu H, Wang J, et al. Glycyrrhizin treatment is associated with attenuation of lipopolysaccharide-induced acute lung injury by inhibiting cyclooxygenase-2 and inducible nitric oxide synthase expression. J Surg Res. 2011;165:e29-35 pubmed publisher
Wang J, Du J, Wang Y, Kuang X, Wang C. Z-ligustilide attenuates lipopolysaccharide-induced proinflammatory response via inhibiting NF-kappaB pathway in primary rat microglia. Acta Pharmacol Sin. 2010;31:791-7 pubmed publisher
Tu X, Yang W, Shi S, Wang C, Zhang G, Ni T, et al. Spatio-temporal distribution of inflammatory reaction and expression of TLR2/4 signaling pathway in rat brain following permanent focal cerebral ischemia. Neurochem Res. 2010;35:1147-55 pubmed publisher

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product information

Product Name :

Anti-COX2/Cyclooxygenase 2 Antibody

Catalog Number :

PA1211

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Prostaglandin G/H synthase 2(PTGS2) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, rat Predicted to work with: mouse

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the N-terminus of human COX2(26-41aa CQNRGVCMSVGFDQYK), different from the related rat sequence by two amino acids.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

Cyclooxygenase(Cox) is the key enzyme in conversion of arachidonic acid to PGs, and two isoforms, Cox-1 and Cox-2, have been identified. Cox-2 gene encodes an inducible prostaglandin synthase enzyme that is overexpressed in adenocarcinomas and other tumors. Deletion of the murine Cox-2 gene in Min mice reduced the incidence of intestinal tumors, suggesting that it is required for tumorigenesis. This gene is localized to sites associated with retinal blood vessels, and plays an important role in blood vessel formation in the retina. And the glucocorticoid receptor suppression of COX-2 is also crucial for curtailing lethal immune activation, and suggest new therapeutic approaches for regulation of T-cell-mediated inflammatory diseases.

Reference :

1. Salmenkivi, K.; Haglund, C.; Ristimaki, A.; Arola, J.; Heikkila, P. : Increased expression of cyclooxygenase-2 in malignant pheochromocytomas. J. Clin. Endocr. Metab. 86: 5615-5619, 2001. 2. Liu, C. H.; Chang, S.-H.; Narko, K.; Trifan, O. C.; Wu, M.-T.; Smith, E.; Haudenschild, C.; Lane, T. F.; Hla, T. : Overexpression of cyclooxygenase-2 is sufficient to induce tumorigenesis in transgenic mice. J. Biol. Chem. 276: 18563-18569, 2001. 3. Wilkinson-Berka, J. L.; Alousis, N. S.; Kelly, D. J.; Gilbert, R. E. : COX-2 inhibition and retinal angiogenesis in a mouse model of retinopathy of prematurity. Invest. Ophthal. Vis. Sci. 44: 974-979, 2003. 4. Brewer, J. A.; Khor, B.; Vogt, S. K.; Muglia, L. M.; Fujiwara, H.; Haegele, K. E.; Sleckman, B. P.; Muglia, L. J. : T-cell glucocorticoid receptor is required to suppress COX-2-mediated lethal immune activation. Nature Med. 9: 1318-1322, 2003.

Gene Name :

PTGS2

Protein Name :

Prostaglandin G/H synthase 2

Gene Full Name :

prostaglandin-endoperoxide synthase 2(prostaglandin G/H synthase and cyclooxygenase)

Synonyms :

COX 2 antibody COX-2 antibody COX2 antibody Cyclooxygenase 2 antibody Cyclooxygenase 2b antibody Cyclooxygenase antibody Cyclooxygenase-2 antibody Cyclooxygenase2 antibody EC 1.14.99.1 antibody fj02a10 antibody Glucocorticoid-regulated inflammatory cyclooxygenase antibody Glucocorticoid-regulated inflammatory Prostaglandin G/H synthase antibody GRIPGHS antibody hCox 2 antibody Macrophage activation-associated marker protein P71/73 antibody OTTHUMP00000033524 antibody PES-2 antibody PGG/HS antibody PGH synthase 2 antibody PGH2_HUMAN antibody PGHS 2 antibody PGHS-2 antibody PGHS2 antibody PHS 2 antibody PHS II antibody PHS2 antibody Prostaglandin endoperoxide synthase 2(prostaglandin G/H synthase and cyclooxygenase) antibody Prostaglandin endoperoxide synthase 2 antibody Prostaglandin G/H synthase 2 antibody Prostaglandin G/H synthase 2 precursor antibody Prostaglandin G/H synthase and cyclooxygenase antibody Prostaglandin G/H synthase antibody Prostaglandin H2 synthase 2 antibody prostaglandin-endoperoxide synthase 2(prostaglandin G/H synthase and cyclooxygenase antibody Prostaglandin-endoperoxide synthase 2 antibody PTGS2 antibody ptgs2a antibody TIS10 antibody TIS10 protein antibody unp1239 antibody wu:fj02a10 antibody

Uniprot ID :

P35354

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