Anti-TNF Receptor I Antibody | Boster Immunoleader PA1210 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-TNF Receptor I Antibody

catalog :

PA1210

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human

application :

western blot, immunohistochemistry - paraffin section

more info or order :

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product information

Product Name :

Anti-TNF Receptor I Antibody

Catalog Number :

PA1210

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Tumor necrosis factor receptor superfamily member 1A(TNFRSF1A) detection. Tested with WB, IHC-P in Human.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence in the middle region of human TNF Receptor I(195-211aa CLPQIENVKGTEDSGTT) .

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

Tumor necrosis factor receptor 1(TNFR1), a potent cytokine, elicits a broad spectrum of biologic responses which are mediated by binding to a cell surface receptor. Its gene is located on 12p13.2. The coding region and the 3-prime untranslated region of TNFR1 are distributed over 10 exons. There are 2 different proteins that serve as major receptors for TNF-alpha, one associated with myeloid cells and one associated with epithelial cells. Additionally, TNFR1 associates with the MADD protein through a death domain-death domain interaction. MADD provides a physical link between TNFR1 and the induction of mitogen-activated protein(MAP) kinase(e.g., ERK2) activation and arachidonic acid release. TNFR1-induced apoptosis involves 2 sequential signaling complexes. Complex I, the initial plasma membrane-bound complex, consists of TNFR1, the adaptor TRADD, the kinase RIP1, and TRAF2 and rapidly signals activation of NF-kappa-B. In a second step, TRADD and RIP1 associate with FADD and caspase-8, forming a cytoplasmic complex, complex II.

Reference :

1. Fuchs, P.; Strehl, S.; Dworzak, M.; Himmler, A.; Ambros, P. F. : Structure of the human TNF receptor 1 (p60) gene (TNFR1) and localization to chromosome 12p13. Genomics 13: 219-224, 1992. 2. Hohmann, H.-P.; Remy, R.; Brockhaus, M.; van Loon, A. P. G. M. : Two different cell types have different major receptors for human tumor necrosis factor (TNF-alpha). J. Biol. Chem. 264: 14927-14934, 1989. 3. Schievella, A. R.; Chen, J. H.; Graham, J. R.; Lin, L.-L. : MADD, a novel death domain protein that interacts with the type 1 tumor necrosis factor receptor and activates mitogen-activated protein kinase. J. Biol. Chem. 272: 12069-12075, 1997. 4. Micheau, O.; Tschopp, J. : Induction of TNF receptor I-mediated apoptosis via two sequential signaling complexes. Cell 114: 181-190, 2003.

Gene Name :

TNFRSF1A

Protein Name :

Tumor necrosis factor receptor superfamily member 1A

Gene Full Name :

tumor necrosis factor receptor superfamily, member 1A

Synonyms :

CD120a antibody FPF antibody MGC19588 antibody p55 antibody p55 R antibody P55R antibody p60 antibody TBP 1 antibody TBP1 antibody TBPI antibody TNF R antibody TNF R I antibody TNF R55 antibody TNF-R1 antibody TNF-RI antibody TNFAR antibody TNFR 55 antibody TNFR 60 antibody TNFR antibody TNFR-I antibody TNFR1 antibody TNFR55 antibody TNFR60 antibody TNFRI antibody TNFRSF1A antibody TNR1A_HUMAN antibody Tumor necrosis factor alpha receptor antibody Tumor necrosis factor binding protein 1 antibody Tumor necrosis factor receptor 1 antibody Tumor necrosis factor receptor superfamily member 1A antibody Tumor necrosis factor receptor type 1 antibody Tumor necrosis factor receptor type I antibody Tumor necrosis factor-binding protein 1 antibody

Uniprot ID :

P19438

more info or order :

Boster Immunoleader product webpage