Anti-Topoisomerase II alpha Antibody | Boster Immunoleader PA1127 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Topoisomerase II alpha Antibody

catalog :

PA1127

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human

application :

western blot

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Topoisomerase II alpha Antibody

Catalog Number :

PA1127

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for DNA topoisomerase 2-alpha(TOP2A) detection. Tested with WB in Human.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human

Applications :

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human Topoisomerase II alpha(1466-1480aa RKPSTSDDSDSNFEK), different from the related rat sequence by three amino acids, and different from the related mouse sequence by four amino acids.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Background :

The human topoisomerase II enzyme is encoded by a single-copy gene which is mapped to 17q21-q22. The TOP2A gene spans approximately 30 kb and contains 35 exons. Furthermore, DNA topoisomerases are enzymes that control and alter the topologic states of DNA in both prokaryotes and eukaryotes. Topoisomerase II from eukaryotic cells catalyzes the relaxation of supercoiled DNA molecules, catenation, decatenation, knotting, and unknotting of circular DNA. It appears likely that the reaction catalyzed by topoisomerase II involves the crossing-over of 2 DNA segments. There are about 100,000 molecules of topoisomerase II per HeLa cell nucleus, constituting about 0.1% of the nuclear extract. DNA topoisomerase II-alpha is associated with the pol II holoenzyme and is a required component of chromatin-dependent coactivation. Specific inhibitors of topoisomerase II blocked transcription on chromatin templates, but did not affect transcription on naked templates. Addition of purified topoisomerase II-alpha reconstituted chromatin-dependent activation activity in reactions with core pol II.

Reference :

1. Miller, K. G.; Liu, L. F.; Englund, P. T. : A homogeneous type II DNA topoisomerase from HeLa cell nuclei. J. Biol. Chem. 256: 9334-9339, 1981. 2. Mondal, N.; Parvin, J. D. : DNA topoisomerase II-alpha is required for RNA polymerase II transcription on chromatin templates. Nature 413: 435-438, 2001.

Gene Name :

TOP2A

Protein Name :

DNA topoisomerase 2-alpha

Gene Full Name :

topoisomerase(DNA) II alpha 170kDa

Synonyms :

alpha isozyme antibody ATP hydrolyzing DNA topoisomerase II alfa antibody DNA gyrase antibody DNA topoisomerase 2 alpha antibody DNA topoisomerase 2-alpha antibody DNA topoisomerase II 170 kD antibody DNA topoisomerase II alpha isozyme antibody DNA topoisomerase II antibody DNA Topoisomerase2 antibody TOP 2A antibody TOP2 antibody TOP2A antibody TOP2A_HUMAN antibody TopII alpha antibody Topo II alpha antibody Topoisomerase DNA II alpha 170kDa antibody Topoisomerase II alpha 170 kDa antibody TP2A antibody

Uniprot ID :

P11388

more info or order :

Boster Immunoleader product webpage