Boster Immunoleader

antibody

Anti-NF-kB p65 Antibody

PA1125

100 ug/vial

200 USD

polyclonal

rabbit

human, mouse, rat

total: 11

1. Liu L, Shang Y, Li M, Han X, Wang J, Wang J. Curcumin ameliorates asthmatic airway inflammation by activating nuclear factor-E2-related factor 2/haem oxygenase (HO)-1 signalling pathway. Clin Exp Pharmacol Physiol. 2015;42:520-9 pubmed publisher
2. Liu M, Su M, Zhang W, Wang Y, Qin L, Liu X, et al. Effect of Melilotus suaveolens extract on pulmonary microvascular permeability by downregulating vascular endothelial growth factor expression in rats with sepsis. Mol Med Rep. 2015;11:3308-16 pubmed publisher
3. Liu W, Shan L, Dong X, Liu Z. Toll-like receptor 4 implicated in acute lung injury induced by paraquat poisoning in mice. Int J Clin Exp Med. 2014;7:3392-7 pubmed
4. Cui L, Feng L, Zhang Z, Jia X. The anti-inflammation effect of baicalin on experimental colitis through inhibiting TLR4/NF-κB pathway activation. Int Immunopharmacol. 2014;23:294-303 pubmed publisher
5. Wang J, Du J, Wang Y, Kuang X, Wang C. Z-ligustilide attenuates lipopolysaccharide-induced proinflammatory response via inhibiting NF-kappaB pathway in primary rat microglia. Acta Pharmacol Sin. 2010;31:791-7 pubmed publisher
6. Ni Y, Wang J, Yan X, Tian F, Zhao J, Wang Y, et al. Histone deacetylase inhibitor, butyrate, attenuates lipopolysaccharide-induced acute lung injury in mice. Respir Res. 2010;11:33 pubmed publisher
7. Ye X, Wu Y, Guo P, Wang J, Yuan S, Shang Y, et al. Lipoxin A4 analogue protects brain and reduces inflammation in a rat model of focal cerebral ischemia reperfusion. Brain Res. 2010;1323:174-83 pubmed publisher
8. Yang M, Xiao C, Wu Q, Niu M, Yao Q, Li K, et al. Anti-inflammatory effect of Sanshuibaihu decoction may be associated with nuclear factor-kappa B and p38 MAPK alpha in collagen-induced arthritis in rat. J Ethnopharmacol. 2010;127:264-73 pubmed publisher
9. Wang L, Zheng X, Xiang H, Fu X, Cao J. 7-Difluoromethyl-5,4'-dimethoxygenistein inhibits oxidative stress induced adhesion between endothelial cells and monocytes via NF-kappaB. Eur J Pharmacol. 2009;605:31-5 pubmed publisher
10. He J, Yu S, Zhu H, Wu J, Qin Z. Triptolide inhibits NF-kappaB activation and reduces injury of donor lung induced by ischemia/reperfusion. Acta Pharmacol Sin. 2007;28:1919-23 pubmed

Anti-NF-kB p65 Antibody

PA1125

Polyclonal

Rabbit IgG polyclonal antibody for Transcription factor p65(RELA) detection. Tested with WB in Human;Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human Predicted to work with: mouse, rat

WB

Concentration: 0.1-0.5μg/ml; Tested Species: Human; Predicted Species: Mouse, Rat

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the N-terminus of human NF-kB p65(116-131aa AISQRIQTNNNPFQVP), identical to the related rat sequence, and different from the related mouse sequence by one amino acid.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

The p65(RELA) heterodimer is the most abundant form of NFKB. This gene is located on 11q13, which consists of 10 exons and spans about 8.1 kb of DNA. In rat sciatic nerves, the expression of the activated p65 subunit of NFKB was high in the nuclei of premyelinating Schwann cells and then progressively declined until it was nearly absent in adults. The transcriptional activity of NF-kappa-B is stimulated upon phosphorylation of its p65 subunit on serine-276 by protein kinase A(PKA). The transcriptional coactivator CBP/p300 associates with NF-kappa-B p65 through 2 sites, an N-terminal domain that interacts with the C-terminal region of unphosphorylated p65, and a second domain that only interacts with p65 phosphorylated on serine-276.

1. Deloukas, P.; van Loon, A. P. G. M. : Genomic organization of the gene encoding the p65 subunit of NF-kappa-B: multiple variants of the p65 protein may be generated by alternative splicing. Hum. Molec. Genet. 2: 1895-1900, 1993. 2. Nickols, J. C.; Valentine, W.; Kanwal, S.; Carter, B. D. : Activation of the transcription factor NF-kappa-B in Schwann cells is required for peripheral myelin formation. Nature Neurosci. 6: 161-167, 2003. 3. Zhong, H.; Voll, R. E.; Ghosh, S. : Phosphorylation of NF-kappa B by PKA stimulates transcriptional activity by promoting a novel bivalent interaction with the coactivator CBP/p300. Molec. Cell 1: 661-671, 1998.

RELA

Transcription factor p65

v-rel reticuloendotheliosis viral oncogene homolog A(avian)

Avian reticuloendotheliosis viral(v rel) oncogene homolog A antibody MGC131774 antibody NF kappa B p65delta3 antibody NFKB 3 antibody NFKB3 antibody Nuclear Factor NF Kappa B p65 Subunit antibody Nuclear factor NF-kappa-B p65 subunit antibody Nuclear factor of kappa light polypeptide gene enhancer in B cells 3 antibody Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B Cells antibody Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 antibody OTTHUMP00000233473 antibody OTTHUMP00000233474 antibody OTTHUMP00000233475 antibody OTTHUMP00000233476 antibody OTTHUMP00000233900 antibody p65 antibody p65 NF kappaB antibody p65 NFkB antibody RELA antibody TF65_HUMAN antibody Transcription Factor p65 antibody V rel avian reticuloendotheliosis viral oncogene homolog A(nuclear factor of kappa light polypeptide gene enhancer in B cells 3(p65)) antibody V Rel Avian Reticuloendotheliosis Viral Oncogene Homolog A antibody v rel reticuloendotheliosis viral oncogene homolog A(avian) antibody V rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappa light polypeptide gene enhancer in B cells 3, p65 antibody v-rel reticuloendotheliosis viral oncogene homolog A antibody

Q04206