Boster Immunoleader

antibody

Anti-HSP60 Antibody

PA1106

100 ug/vial

200 USD

polyclonal

rabbit

human, mouse, rat

western blot, immunocytochemistry, immunohistochemistry - paraffin section

Anti-HSP60 Antibody

PA1106

Polyclonal

Rabbit IgG polyclonal antibody for 60 kDa heat shock protein, mitochondrial(HSPD1) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, mouse, rat

WB,IHC-P,ICC

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Concentration: 0.5-1μg/ml; Tested Species: Human, Mouse, Rat

Concentration: 0.5-1μg/ml; Tested Species: Human

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the C-terminus of rat HSP60(503-516aa YDAMLGDFVNMVEK), different from the related human sequence by one amino acid.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P) and ICC.

HSP60 is a member of the chaperonin class of protein factors, which include the Escherichia coli groEL protein and the Rubisco subunit-binding protein of chloroplasts. It acts as a costimulator of human regulatory CD4-positive/CD25 -positive T cells, which inhibit lymphoproliferation and IFNG and TNF secretion by CD4-positive and CD8-positive T cells. HSP60 enhances Treg activity via TLR2, leading to activation of an intracellular signaling cascade that included p38, as well as inhibition of ERK phosphorylation. Suppression of target T cells is mediated by both cell-to-cell contact and by secretion of TGFB and IL10, and it leads to downregulation of ERK, NFKB, and TBET expression. The self-molecule HSP60 can downregulate adaptive immune responses by upregulating Tregs through TLR2 signaling.

1. Cheng, M. Y.; Hartl, F.-U.; Martin, J.; Pollock, R. A.; Kalousek, F.; Neupert, W.; Hallberg, E. M.; Hallberg, R. L.; Horwich, A. L. : Mitochondrial heat-shock protein hsp60 is essential for assembly of proteins imported into yeast mitochondria. Nature 337: 620-625, 1989. 2. Zanin-Zhorov, A.; Cahalon, L.; Tal, G.; Margalit, R.; Lider, O.; Cohen, I. R. : Heat shock protein 60 enhances CD4+CD25+ regulatory T cell function via innate TLR2 signaling. J. Clin. Invest. 116: 2022-2032, 2006.

HSPD1

60 kDa heat shock protein, mitochondrial

heat shock 60kDa protein 1(chaperonin)

60 kDa chaperonin antibody 60 kDa heat shock protein mitochondrial antibody 60 kDa heat shock protein, mitochondrial antibody CH60_HUMAN antibody Chaperonin 60 antibody Chaperonin antibody Chaperonin, 60-KD antibody CPN 60 antibody CPN60 antibody fa04a05 antibody GROEL antibody GroEL Homolog antibody Heat shock 60kD protein 1 chaperonin antibody Heat shock 60kDa protein 1 antibody Heat shock protein 1(chaperonin) antibody Heat Shock Protein 60 antibody Heat shock protein 65 antibody HLD4 antibody Hsp 60 antibody HSP 65 antibody HSP-60 antibody HSP60 antibody HSP65 antibody HSPD 1 antibody HSPD1 antibody HuCHA60 antibody Mitochondrial matrix protein P1 antibody P60 lymphocyte protein antibody Short heat shock protein 60 Hsp60s1 antibody Spastic paraplegia 13 antibody SPG 13 antibody SPG13 antibody

P63039