Boster Immunoleader

antibody

Anti-Lamin A/C Antibody

PA1103

100 ug/vial

200 USD

polyclonal

rabbit

human, mouse, rat

western blot, immunocytochemistry, immunohistochemistry - paraffin section

info changed 20150505

Anti-Lamin A/C Antibody

PA1103

Polyclonal

Rabbit IgG polyclonal antibody for Prelamin-A/C(LMNA) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, mouse, rat

WB,IHC-P,ICC

Concentration: 0.1-0.5μg/ml; Tested Species: Human; Predicted Species: Mouse, Rat

Concentration: 0.5-1μg/ml; Tested Species: Human, Mouse, Rat

Concentration: 0.5-1μg/ml; Tested Species: Human; Predicted Species: Mouse, Rat

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the C-terminus of human Lamin A/C(455-469aa RNKSNEDQSMGNWQI), identical to the related rat and mouse sequences.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P) and ICC.

Lamins are structural protein components of the nuclear lamina, a protein network underlying the inner nuclear membrane that determines nuclear shape and size. There are three types of lamins, A,B and C. The lamin A/C(LMNA) gene contains 12 exons. Alternative splicing within exon 10 gives rise to two different mRNAs that code for pre-lamin A and lamin C. Lamin A/C mapped to 1q21.2-q21.3 and mutations in this gene cause a variety of human diseases including Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy, and Hutchinson-Gilford progeria syndrome. Lamin A/C deficiency is thus associated with both defective nuclear mechanics and impaired mechanically activated gene transcription.

1. Lammerding, J.; Schulze, P. C.; Takahashi, T.; Kozlov, S.; Sullivan, T.; Kamm, R. D.; Stewart, C. L.; Lee, R. T. : Lamin A/C deficiency causes defective nuclear mechanics and mechanotransduction. J. Clin. Invest. 113: 370-378, 2004. 2. Lin, F.; Worman, H. J. : Structural organization of the human gene encoding nuclear lamin A and nuclear lamin C. J. Biol. Chem. 268: 16321-16326, 1993. 3. Wydner, K. L.; McNeil, J. A.; Lin, F.; Worman, H. J.; Lawrence, J. B. : Chromosomal assignment of human nuclear envelope protein genes LMNA, LMNB1, and LBR by fluorescence in situ hybridization. Genomics 32: 474-478, 1996.

LMNA

Prelamin-A/C

lamin A/C

70 kDa Lamin antibody Cardiomyopathy dilated 1A(autosomal dominant) antibody CDCD1 antibody CDDC antibody Charcot Marie Tooth Disease Axonal Type 2B1 antibody CMD1A antibody CMT2B1 antibody EMD2 antibody FPL antibody FPLD antibody FPLD2 antibody HGPS antibody IDC antibody Lamin A/C antibody Lamin A/C Isoform 1 Precursor antibody Lamin A/C Isoform 2 antibody Lamin A/C Isoform 3 antibody Lamin A/C like 1 antibody Lamin-A/C antibody LDP1 antibody LFP antibody LGMD1B antibody Limb girdle muscular dystrophy 1B(autosomal dominant) antibody LMN 1 antibody LMN A antibody LMN C antibody LMN1 antibody LMNA antibody LMNA_HUMAN antibody LMNC antibody NY REN 32 antigen antibody Prelamin A/C antibody PRO1 antibody Progeria 1(Hutchinson Gilford Type) antibody Renal carcinoma antigen NY REN 32 antibody Renal carcinoma antigen NY-REN-32 antibody Renal carcinoma antigen NYREN32 antibody

P02545