Anti-Fas Ligand Antibody | Boster Immunoleader PA1101 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Fas Ligand Antibody

catalog :

PA1101

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human

application :

western blot, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Fas Ligand Antibody

Catalog Number :

PA1101

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Tumor necrosis factor ligand superfamily member 6(FASLG) detection. Tested with WB, IHC-P, ICC in Human.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human

Applications :

WB,IHC-P,ICC

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human

Immunocytochemistry :

Concentration: 0.5-1μg/ml; Tested Species: Human

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human Fas Ligand(192-218aa YSKVYFRGQSCNNLPLSHKVYMRNSKY), different from the related mouse sequence by two amino acids, and from the related rat sequence by three amino acids.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P) and ICC.

Background :

FAS Ligand(FASL) is a 40 kDa type II membrane protein belonging to the tumor necrosis factor family, which induces apoptosis by binding to its receptor, Fas. The human FasL gene consists of approximately 8.0 kb and is split into four exons. This gene consists of 281 amino acids with a calculated M(r) of 31,759 and was mapped on chromosome 1q23. It has an identity of 76.9% at the amino acid sequence level with mouse FasL. The FAS and FASL system plays a key role in regulating apoptotic cell death and corruption of this signalling pathway has been shown to participate in immune escape and tumorigenesis. FAS and FASL triggered apoptosis pathway plays an important role in human carcinogenesis. This system may also play a role in modulating the genetic susceptibility of mouse strains to develop T-cell lymphoblastic lymphomas.

Reference :

1. Takahashi, T.; Tanaka, M.; Inazawa, J.; Abe, T.; Suda, T.; Nagata, S. : Human Fas ligand: gene structure, chromosomal location and species specificity. Int. Immun. 6: 1567-1574, 1994. 2. Zhang, X.; Miao, X.; Sun, T.; Tan, W.; Qu, S.; Xiong, P.; Zhou, Y.; Lin, D. : Functional polymorphisms in cell death pathway genes FAS and FASL contribute to the risk of lung cancer. J. Med. Genet. 42: 479-484, 2005. 3. Villa-Morales, M.; Santos, J.; Perez-Gomez, E.; Quintanilla, M.; Fernandez-Piqueras, J. : A role for the Fas/FasL system in modulating genetic susceptibility to T-cell lymphoblastic lymphomas. Cancer Res. 67: 5107-5116, 2007.

Gene Name :

FASLG

Protein Name :

Tumor necrosis factor ligand superfamily member 6

Gene Full Name :

Fas ligand(TNF superfamily, member 6)

Synonyms :

ALPS1B antibody APO1L antibody Apoptosis(APO 1) antigen ligand 1 antibody Apoptosis antigen ligand 1 antibody Apoptosis antigen ligand antibody APT1LG1 antibody APTL antibody CD178 antibody CD178 antigen antibody CD95 ligand antibody CD95-L antibody CD95L antibody CD95L protein antibody FAS antigen ligand antibody Fas L antibody Fas ligand(TNF superfamily member 6) antibody Fas ligand antibody FASL antibody Fasl Fas ligand(TNF superfamily member 6) antibody FASLG antibody Generalized lymphoproliferative disease antibody Gld antibody soluble form antibody TNF superfamily member 6 antibody TNFL6_HUMAN antibody TNFSF6 antibody Tumor necrosis factor(ligand) superfamily member 6 antibody Tumor necrosis factor ligand superfamily member 6 antibody

Uniprot ID :

P48023

more info or order :

Boster Immunoleader product webpage