Boster Immunoleader

antibody

Anti-Orexin Receptor 1 Antibody

PA1100

100 ug/vial

200 USD

polyclonal

rabbit

human, mouse, rat

western blot, immunohistochemistry - paraffin section

Anti-Orexin Receptor 1 Antibody

PA1100

Polyclonal

Rabbit IgG polyclonal antibody for Orexin receptor type 1(HCRTR1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, rat Predicted to work with: mouse

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Concentration: 0.5-1μg/ml; Tested Species: Rat; Predicted Species: Human, Mouse

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the C-terminus of human Orexin Receptor 1(327-342aa FRQASDREAVYACFTF), identical to the related mouse and rat sequences.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Orexins are endogeneous peptides that activate Orexin Receptor type 1 and type 2, two closely related G-protein coupled receptors. Orexins-A and -B are hypothalamic peptides derived from a precursor called preproorexin and affect the stimulation of food intake. They act on G protein receptors named orexin receptor-1(OX1R) and orexin receptor-2(OX2R), respectively. Stimulation of these receptors by orexins causes an increase in intracellular calcium levels in hypothalamic cells in vitro. In vivo, orexins control feeding and may also be involved in modulating sleep-wake cycles and other hypothalamic functions.

1. Blanco, M.; Lopez, M.; Garcia-Caballero, T.; Gallego, R.; Vazquez-Boquete, A.; Morel, G.; Senaris, R.; Casanueva, F.; Dieguez, C.; Beiras, A. : Cellular localization of orexin receptors in human pituitary. J. Clin. Endocr. Metab. 86: 1616-1619, 2001. 2. Sakurai, T.; Amemiya, A.; Ishii, M.; Matsuzaki, I.; Chemelli, R. M.; Tanaka, H.; Williams, S. C.; Richardson, J. A.; Kozlowski, G. P.; Wilson, S.; Arch, J. R. S.; Buckingham, R. E.; Haynes, A. C.; Carr, S. A.; Annan, R. S.; McNulty, D. E.; Liu, W.-S.; Terrett, J. A.; Elshourbagy, N. A.; Bergsma, D. J.; Yanagisawa, M. : Orexins and orexin receptors: a family of hypothalamic neuropeptides and G protein-coupled receptors that regulate feeding behavior. Cell 92: 573-585, 1998.

HCRTR1

Orexin receptor type 1(Ox-1-R/Ox1-R/Ox1R)

hypocretin(orexin) receptor 1

HCRTR 1 antibody HCRTR1 antibody Hypocretin receptor type 1 antibody Orexin receptor type 1 antibody Ox-1-R antibody Ox1-R antibody Ox1r antibody OX1R_HUMAN antibody

O43613