Anti-TIMP2 Antibody | Boster Immunoleader PA1076 product information

product summary

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company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-TIMP2 Antibody

catalog :

PA1076

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

ELISA, immunohistochemistry - paraffin section

publications citing this reagent :

Cheng G, Wei L, Xiurong W, Xiangzhen L, Shiguang Z, Songbin F. IL-17 stimulates migration of carotid artery vascular smooth muscle cells in an MMP-9 dependent manner via p38 MAPK and ERK1/2-dependent NF-kappaB and AP-1 activation. Cell Mol Neurobiol. 2009;29:1161-8 pubmed publisher

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Boster Immunoleader product webpage

product information

Product Name :

Anti-TIMP2 Antibody

Catalog Number :

PA1076

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Metalloproteinase inhibitor 2(TIMP2) detection. Tested with IHC-P, ELISA in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human Predicted to work with: mouse, rat

Applications :

IHC-P,ELISA

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human; Predicted Species: Mouse, Rat

ELISA :

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human TIMP2(205-220aa RGAAPPKQEFLDIEDP), identical to the related mouse and rat sequences.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by SA1022 in IHC(P).

Background :

TIMP2 gen is encoded by 5 exons spanning 83 kb of genomic DNA. TIMP2 is 83 kilobase pairs(kb) long with exon-intron splicing sites located in preserved positions among the three members of the TIMP family. The gene for tissue inhibitor of metalloproteinases-2 is localized on human chromosome arm 17q25. TIMP-2 abrogates angiogenic factor-induced endothelial cell proliferation in vitro and angiogenesis in vivo independent of MMP inhibition.

Reference :

1. De Clerck, Y.; Szpirer, C.; Aly, M. S.; Cassiman, J.-J.; Eeckhout, Y.; Rousseau, G. : The gene for tissue inhibitor of metalloproteinases-2 is localized on human chromosome arm 17q25. Genomics 14: 782-784, 1992. 2. Hammani, K.; Blakis, A.; Morsette, D.; Bowcock, A. M.; Schmutte, C.; Henriet, P.; DeClerck, Y. A. : Structure and characterization of the human tissue inhibitor of metalloproteinases-2 gene. J. Biol. Chem. 271: 25498-25505, 1996. 3. Seo, D.-W.; Li, H.; Guedez, L.; Wingfield, P. T.; Diaz, T.; Salloum, R.; Wei, B.; Stetler-Stevenson, W. G. : TIMP-2 mediated inhibition of angiogenesis: an MMP-independent mechanism. Cell 114: 171-180, 2003.

Gene Name :

TIMP2

Protein Name :

Metalloproteinase inhibitor 2

Gene Full Name :

TIMP metallopeptidase inhibitor 2

Synonyms :

CSC 21K antibody CSC-21K antibody CSC21K antibody Metalloproteinase inhibitor 2 antibody Metalloproteinase inhibitor 2 precursor antibody TIMP 2 antibody TIMP metallopeptidase inhibitor 2 antibody TIMP-2 antibody TIMP2 antibody TIMP2_HUMAN antibody Tissue Inhibitor of Metalloproteinase 2 antibody Tissue inhibitor of metalloproteinases 2 antibody

Uniprot ID :

P16035

more info or order :

Boster Immunoleader product webpage