Anti-Sonic Hedgehog Antibody | Boster Immunoleader PA1072-1 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Sonic Hedgehog Antibody

catalog :

PA1072-1

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Sonic Hedgehog Antibody

Catalog Number :

PA1072-1

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Sonic hedgehog protein(SHH) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, rat Predicted to work with: mouse

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the N-terminus of human Sonic Hedgehog(203-218aa ATVHLEQGGTKLVKDL), identical to the related rat and mouse sequences.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

The mouse, chicken, and zebrafish Shh homologs are highly conserved. SHH expression was not detected in adult tissues examined. However, it was expressed in fetal intestine, liver, lung, and kidney. SHH gene is mapped to 7q. SHH mutations are not a frequent cause of isolated oral clefts.

Reference :

1. Tsukurov, O.; Boehmer, A.; Flynn, J.; Nicolai, J.-P.; Hamel, B. C. J.; Traill, S.; Zaleske, D.; Mankin, H. J.; Yeon, H.; Ho, C.; Tabin, C.; Seidman, J. G.; Seidman, C. : A complex bilateral polysyndactyly disease locus maps to chromosome 7q36. Nature Genet. 6: 282-286, 1994.

Gene Name :

SHH

Protein Name :

Sonic hedgehog protein

Gene Full Name :

sonic hedgehog

Synonyms :

HHG 1 antibody HHG-1 antibody HHG1 antibody HLP 3 antibody HLP3 antibody Holoprosencephaly 3 antibody HPE 3 antibody HPE3 antibody MCOPCB5 antibody SHH antibody SHH_HUMAN antibody SMMC I antibody SMMCI antibody Sonic Hedgehog(Drosophila) homolog antibody sonic hedgehog homolog(Drosophila) antibody Sonic hedgehog homolog antibody Sonic hedgehog protein antibody Sonic hedgehog protein C-product antibody TPT antibody TPTPS antibody

Uniprot ID :

Q15465

more info or order :

Boster Immunoleader product webpage