Anti-NSE Antibody | Boster Immunoleader PA1061 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-NSE Antibody

catalog :

PA1061

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

publications citing this reagent :

Cai Z, Zhang X, Wang G, Wang H, Liu Z, Guo X, et al. BDNF attenuates IL-1β-induced F-actin remodeling by inhibiting NF-κB signaling in hippocampal neurons. Neuro Endocrinol Lett. 2014;35:13-9 pubmed
Lu L, Wang W, Peng Y, Li J, Wang L, Wang X. Electrophysiology and pharmacology of tandem domain potassium channel TREK-1 related BDNF synthesis in rat astrocytes. Naunyn Schmiedebergs Arch Pharmacol. 2014;387:303-12 pubmed publisher
Sun C, Shao J, Su L, Zhao J, Bi J, Yang S, et al. Cholinergic neuron-like cells derived from bone marrow stromal cells induced by tricyclodecane-9-yl-xanthogenate promote functional recovery and neural protection after spinal cord injury. Cell Transplant. 2013;22:961-75 pubmed publisher

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-NSE Antibody

Catalog Number :

PA1061

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Gamma-enolase(ENO2) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: rat Predicted to work with: human, mouse

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Rat; Predicted Species: Human, Mouse

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Rat; Predicted Species: Human, Mouse

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human NSE(420-434aa EARFAGHNFRNPSVL), identical to the related mouse and rat sequences.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

NSE(neuron specific enolase), also known as Enolase 2(ENO2), is found in elevated concentrations in plasma in certain neoplasias. The enolases catalyze the interconversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway. ENO2 gene contains 12 exons distributed over 9,213 nucleotides. Human neurone-specific enolase is mapped to chromosome 12p13.

Reference :

1. Craig, S. P.; Day, I. N. M.; Thompson, R. J.; Craig, I. W. : Localization of human neurone-specific enolase to chromosome 12p13. (Abstract) Cytogenet. Cell Genet. 51: 980 only, 1989. 2. Craig, S. P.; Day, I. N. M.; Thompson, R. J.; Craig, I. W. : Localisation of neurone-specific enolase (ENO2) to 12p13. Cytogenet. Cell Genet. 54: 71-73, 1990. 3. Mattei, J. F.; Baeteman, M. A.; Mattei, M. G.; Ardissonne, J. P.; Giraud, F. : Regional assignments of CS and ENO2 on chromosome 12. (Abstract) Cytogenet. Cell Genet. 32: 297 only, 1982.

Gene Name :

ENO2

Protein Name :

Gamma-enolase

Gene Full Name :

enolase 2(gamma, neuronal)

Synonyms :

2 phospho D glycerate hydrolyase antibody 2-phospho-D-glycerate hydro-lyase antibody Eno 2 antibody Eno2 antibody ENOG antibody ENOG_HUMAN antibody Enolase 2 antibody Enolase 2 gamma neuronal antibody Enolase2 antibody Gamma enolase antibody Gamma-enolase antibody Neural enolase antibody Neuron specific enolase antibody Neuron specific gamma enolase antibody Neuron-specific enolase antibody Neurone specific enolase antibody NSE antibody

Uniprot ID :

P09104

more info or order :

Boster Immunoleader product webpage