Anti-Nogo A Antibody | Boster Immunoleader PA1060 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Nogo A Antibody

catalog :

PA1060

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Nogo A Antibody

Catalog Number :

PA1060

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Reticulon-4(RTN4) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: mouse, rat Predicted to work with: human

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Mouse, Rat; Predicted Species: Human

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Rat; Predicted Species: Human, Mouse

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human Nogo A(1170-1192aa NKNVKDAMAKIQAKIPGLKRKAE), different from the related rat sequence by two amino acids, and from the related mouse sequence by one amino acid.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

Human neurite outgrowth inhibitor(NOGO) cDNAs encodes 3 splice variants: NOGOA, NOGOB and NOGOC. The longest cDNA, designated NOGOA, has an open reading frame of 1192 amino acids. It is a potent inhibitor of neurite growth and an IN-1 antigen produced by oligodendrocytes, and may allow the generation of new reagents to enhance CNS regeneration and plasticity. Nogo-A, a member of the Reticulon family, is expressed by oligodendrocytes and associates primarily with the endoplasmic reticulum. The acidic amino terminus of Nogo-A is detected at the cytosolic face of cellular membranes and may contribute to inhibition of axon regeneration at sites of oligodendrocyte injury. A multivalent form of the N terminus of Nogo-A affects the morphology of both neurons and other cell types.

Reference :

1. Prinjha R, Moore SE, Vinson M, Blake S, Morrow R, Christie G, Michalovich D, Simmons DL, Walsh FS.Inhibitor of neurite outgrowth in humans. Nature. 2000 Jan 27; 403(6768):383-4. 2. Chen MS, Huber AB, van der Haar ME, Frank M, Schnell L, Spillmann AA, Christ F, Schwab ME.Nogo-A is a myelin-associated neurite outgrowth inhibitor and an antigen for monoclonal antibody IN-1. Nature. 2000 Jan 27; 403(6768):434-9. 3. GrandPre T, Nakamura F, Vartanian T, Strittmatter SM.Identification of the Nogo inhibitor of axon regeneration as a Reticulon protein. Nature. 2000 Jan 27; 403(6768):439-44. 4. Fournier AE, GrandPre T, Strittmatter SM. Identification of a receptor mediating Nogo-66 inhibition of axonal regeneration. Nature. 2001 Jan 18; 409(6818):341-6.

Gene Name :

RTN4

Protein Name :

Reticulon-4

Gene Full Name :

reticulon 4

Synonyms :

ASY antibody Foocen antibody Human NogoA antibody KIAA0886 antibody My043 protein antibody Nbla00271 antibody Nbla10545 antibody Neurite growth inhibitor 220 antibody Neurite Outgrowth Inhibitor antibody Neuroendocrine specific protein antibody Neuroendocrine specific protein C homolog antibody Neuroendocrine-specific protein antibody Neuroendocrine-specific protein C homolog antibody NI220/250 antibody Nogo A antibody NOGO antibody Nogo protein antibody NOGO-A antibody Nogo-B antibody Nogo-C antibody NogoA antibody NSP antibody NSP CL antibody NSP-CL antibody Reticulon 4 antibody Reticulon 5 antibody Reticulon-4 antibody Reticulon-5 antibody Reticulon4 antibody Reticulon5 antibody RTN 4 antibody RTN 4A antibody RTN X antibody RTN xL antibody RTN-x antibody RTN4 A antibody RTN4 antibody RTN4 B1 antibody RTN4 B2 antibody RTN4 C antibody RTN4 protein antibody RTN4-A antibody RTN4-B1 antibody RTN4-B2 antibody RTN4-C antibody RTN4_HUMAN antibody

Uniprot ID :

Q9NQC3

more info or order :

Boster Immunoleader product webpage