Anti-MIF Antibody | Boster Immunoleader PA1052 product information

product summary

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company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-MIF Antibody

catalog :

PA1052

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

publications citing this reagent :

Wang Y, Tian F, Liu H, Zhang W, Li J, Xiao B, et al. Macrophage migration inhibitory factor is necessary for the lipo-oligosaccharide-induced response by modulation of Toll-like receptor 4 in monocytes from GBS patients. J Neuroimmunol. 2013;257:67-75 pubmed publisher

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product information

Availability :

info changed 20140620

Product Name :

Anti-MIF Antibody

Catalog Number :

PA1052

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Macrophage migration inhibitory factor(MIF) detection. Tested with WB,IHC-P in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, rat Predicted to work with: mouse

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human; Predicted Species: Mouse, Rat

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human MIF(98-115aa NYYDMNAANVGWNNSTFA), different from the related rat and mouse sequences by one amino acid.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

Macrophage migration inhibitory factor, MIF, is a cytokine released by T-lymphocytes, macrophages, and the pituitary gland that serves to integrate peripheral and central inflammatory responses. MIF gene has 3 exons separated by introns of only 189 and 95 bp, and covers less than 1 kb. Localization of the human gene for macrophage migration inhibitory factor(MIF) to chromosome 22q11.2 MIF plays a critical role in inflammatory diseases and atherogenesis.

Reference :

1. Bozza, M.; Kolakowski, L. F., Jr.; Jenkins, N. A.; Gilbert, D. J.; Copeland, N. G.; David, J. R.; Gerard, C. : Structural characterization and chromosomal location of the mouse macrophage migration inhibitory factor gene and pseudogenes. Genomics 27: 412-419, 1995 2. Budarf, M.; McDonald, T.; Sellinger, B.; Kozak, C.; Graham, C.; Wistow, G. : Localization of the human gene for macrophage migration inhibitory factor (MIF) to chromosome 22q11.2. Genomics 39: 235-236, 1997. 3. Bernhagen, J.; Krohn, R.; Lue, H.; Gregory, J. L.; Zernecke, A.; Koenen, R. R.; Dewor, M.; Georgiev, I.; Schober, A.; Leng, L.; Kooistra, T.; Fingerle-Rowson, G.; Ghezzi, P.; Kleemann, R.; McColl, S. R.; Bucala, R.; Hickey, M. J.; Weber, C. : MIF is a noncognate ligand of CXC chemokine receptors in inflammatory and atherogenic cell recruitment. Nature Med. 13: 587-596, 2007.

Gene Name :

MIF

Protein Name :

Macrophage migration inhibitory factor(MIF)

Gene Full Name :

macrophage migration inhibitory factor(glycosylation-inhibiting factor)

Synonyms :

acrophage migration inhibitory factor(glycosylation-inhibiting factor) antibody GIF antibody GLIF antibody Glycosylation inhibiting factor antibody Glycosylation-inhibiting factor antibody L-dopachrome isomerase antibody L-dopachrome tautomerase antibody Macrophage migration inhibitory factor antibody MIF antibody MIF protein antibody MMIF antibody Phenylpyruvate tautomerase antibody

Uniprot ID :

P14174

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