Boster Immunoleader

antibody

Anti-Mitofusin 2 Antibody

PA1051

100 ug/vial

200 USD

polyclonal

rabbit

human, mouse, rat

Anti-Mitofusin 2 Antibody

PA1051

Polyclonal

Rabbit IgG polyclonal antibody for Mitofusin-2(MFN2) detection. Tested with WB in Human;Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, rat Predicted to work with: mouse

WB

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the N-terminus of human Mitofusin 2(6-24aa SRCNSIVTVKKNKRHMAEV), different from the related rat and mouse sequences by one amino acid.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Mitofusin 2(MFN2) is a mitochondrial transmembrane GTPase regulating mitochondrial fusion and that the nucleotide-dependent activation of MFN2 concomitantly protects the organelle from permeability transition. It is mapped to chromosome 1 and encodes a 757-amino acid protein that contains an ATP/GTP-binding site motif. It is expressed in many tissues and cell lines such as brain and KG-1 with the highest expression in heart and skeletal muscle. This protein contains an N-terminal GTPase domain and a transmembrane domain near the C terminus. It shares 60% identity with MFN1. When stably expressed in COS-7 cells, MFN2 colocalizes with mitochondrial markers. Axonal CMT type 2A and autosomal dominant HMSN VI are caused by MFN2 and mutations in MFN2, which emphasizes its important role of mitochondrial function for both optic atrophies and peripheral neuropathies.

1. Neuspiel M, Zunino R, Gangaraju S, Rippstein P, McBride H. Activated mitofusin 2 signals mitochondrial fusion, interferes with Bax activation, and reduces susceptibility to radical induced depolarization. J Biol Chem. 2005 Jul 1; 280(26):25060-70. 2. Nagase T, Seki N, Ishikawa K, Ohira M, Kawarabayasi Y, Ohara O, Tanaka A, Kotani H, Miyajima N, Nomura N. Prediction of the coding sequences of unidentified human genes. VI. The coding sequences of 80 new genes (KIAA0201-KIAA0280) deduced by analysis of cDNA clones from cell line KG-1 and brain. DNA Res. 1996 Oct 31; 3(5):321-9, 341-54. 3. Santel A, Fuller MT. Control of mitochondrial morphology by a human mitofusin. J Cell Sci. 2001 Mar; 114(Pt 5):867-74. 4. Zuchner S, De Jonghe P, Jordanova A, Claeys KG, Guergueltcheva V, Cherninkova S, Hamilton SR, Van Stavern G, Krajewski KM, Stajich J, Tournev I, Verhoeven K, Langerhorst CT, de Visser M, Baas F, Bird T, Timmerman V, Shy M, Vance JM. Axonal neuropathy with optic atrophy is caused by mutations in mitofusin 2. Ann Neurol. 2006 Feb; 59(2):276.

MFN2

Mitofusin-2

mitofusin 2

CMT2A antibody CMT2A2 antibody CPRP 1 antibody CPRP1 antibody EC 3.6.5.- antibody Fzo antibody HSG antibody hyperplasia suppressor gene antibody Hypertension related protein 1 antibody KIAA0214 antibody MARF antibody MFN 2 antibody MFN2 antibody MFN2_HUMAN antibody Mitochondrial assembly regulatory factor antibody Mitofusin-2 antibody Mitofusin2 antibody Transmembrane GTPase MFN2 antibody

O95140