Anti-Myelin Basic Protein Antibody | Boster Immunoleader PA1050 product information

product summary

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company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Myelin Basic Protein Antibody

catalog :

PA1050

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse

application :

western blot, immunohistochemistry - paraffin section

publications citing this reagent :

Song J, Wu C, Korpos E, Zhang X, Agrawal S, Wang Y, et al. Focal MMP-2 and MMP-9 activity at the blood-brain barrier promotes chemokine-induced leukocyte migration. Cell Rep. 2015;10:1040-54 pubmed publisher
Chen X, Chen X, Huang X, Qin C, Fang Y, Liu Y, et al. Soluble epoxide hydrolase inhibition provides multi-target therapeutic effects in rats after spinal cord injury. Mol Neurobiol. 2015;: pubmed

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product information

Product Name :

Anti-Myelin Basic Protein Antibody

Catalog Number :

PA1050

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Myelin basic protein(MBP) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, mouse, rat

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Mouse, Rat

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Mouse, Rat; Predicted Species: Human

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human Myelin Basic Protein(182-197aa KLGGRDSRSGSPMARR), identical to the related rat and mouse sequences.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

Myelin basic protein(MBP) is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the central nervous system and the peripheral nervous system, respectively. It is most abundant in hemopoietic system and contains seven exons distributed over 32-34 kb. MBP isolated from MS brain may differ in charge microheterogeneity which would affect antigenic determinants. MBP is mapped to chromosome 18q22-23. Failure in this gene expression would be correlated in the central white matter with extrapyramidal system degeneration signs. Moreover, it is a candidate autoantigen in the disease multiple sclerosis.

Reference :

1. Marty MC, Alliot F, Rutin J, Fritz R, Trisler D, Pessac B.The myelin basic protein gene is expressed in differentiated blood cell lineages and in hemopoietic progenitors. Proc Natl Acad Sci U S A. 2002 Jun 25; 99(13):8856-61. 2. Streicher R, Stoffel W.The organization of the human myelin basic protein gene. Comparison with the mouse gene. Biol Chem Hoppe Seyler. 1989 May; 370(5):503-10. 3. McLaurin JA, Hafler DA, Antel JP.Reactivity of normal T-cell lines to MBP isolated from normal and multiple sclerosis white matter. J Neurol Sci. 1995 Feb; 128(2):205-11. 4. Iester A, Vignola S, Callegarini L, Gimelli G, Alpigiani MG. [18q syndrome with deficiency of myelin basic protein (MBP)] Pediatr Med Chir. 1996 Mar-Apr; 18(2):201-5. 5. Tranquill LR, Skinner E, Campagnoni C, Vergelli M, Hemmer B, Muraro P, Martin R, McFarland HF, Campagnoni AT, Voskuhl RR. Human T lymphocytes specific for the immunodominant 83-99 epitope of myelin basic protein: recognition of golli MBP HOG 7. J Neurosci Res. 1996 Sep 15; 45(6):820-8.

Gene Name :

MBP

Protein Name :

Myelin basic protein(MBP)

Gene Full Name :

myelin basic protein

Synonyms :

GDB antibody Golli MBP antibody Hemopoietic MBP antibody HMBPR antibody HUGO antibody MBP antibody MBP_HUMAN antibody MGC99675 antibody MLD antibody Myelin A1 Protein antibody Myelin basic protein antibody Myelin Deficient antibody Myelin Membrane Encephalitogenic Protein antibody OTTHUMP00000163776 antibody OTTHUMP00000174387 antibody OTTHUMP00000174388 antibody SHI antibody Shiverer antibody SP antibody

Uniprot ID :

P02686

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