Anti-Lamin B1 Antibody | Boster Immunoleader PA1048 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Lamin B1 Antibody

catalog :

PA1048

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, rat

application :

western blot, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

publications citing this reagent :

Ni Y, Wang J, Yan X, Tian F, Zhao J, Wang Y, et al. Histone deacetylase inhibitor, butyrate, attenuates lipopolysaccharide-induced acute lung injury in mice. Respir Res. 2010;11:33 pubmed publisher

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Lamin B1 Antibody

Catalog Number :

PA1048

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Lamin-B1(LMNB1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, rat

Applications :

WB,IHC-P,IHC-F,ICC

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human, Rat

Immunohistochemistry(Frozen Section) :

Concentration: 0.5-1μg/ml; Tested Species: Rat; Predicted Species: Human

Immunocytochemistry :

Concentration: 0.5-1μg/ml; Tested Species: Human; Predicted Species: Rat

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staning of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human Lamin B1(570-586aa FHQQGTPRASNRSCAIM), different from the related rat sequence by one amino acid, and from the related mouse sequence by three amino acids.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P), IHC(F) and ICC.

Background :

Lamin-B1 is a protein that in humans is encoded by the LMNB1 gene. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are though to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1.

Reference :

1. Pollard, K. M.; Chan, E. K.; Grant, B. J.; Sullivan, K. E.; Tan, E. M.; Glass, C. A. : In vitro posttranslational modification of lamin B clones from a human T-cell line. Molec. Cell. Biol. 10: 2164-2175, 1990 2. Tsai, M.-Y.; Wang, S.; Heidinger, J. M.; Shumaker, D. K.; Adam, S. A.; Goldman, R. D.; Zheng, Y. : A mitotic lamin B matrix induced by RanGTP required for spindle assembly. Science 311: 1887-1893, 2006.

Gene Name :

LMNB1

Protein Name :

Lamin-B1

Gene Full Name :

lamin B1

Synonyms :

ADLD antibody lamin B1 antibody Lamin-B1 antibody LMN antibody LMN2 antibody LMNB antibody Lmnb1 antibody LMNB1_HUMAN antibody MGC111419 antibody OTTHUMP00000159218 antibody

Uniprot ID :

P20700

more info or order :

Boster Immunoleader product webpage