Boster Immunoleader

antibody

Anti-INSL3 Antibody

PA1044

100 ug/vial

200 USD

polyclonal

rabbit

human, mouse, rat

western blot, immunohistochemistry - paraffin section

Anti-INSL3 Antibody

PA1044

Polyclonal

Rabbit IgG polyclonal antibody for Insulin-like 3(INSL3) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, rat Predicted to work with: mouse

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Rat; Predicted Species: Human, Mouse

Concentration: 0.5-1μg/ml; Tested Species: Human

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the C-terminus of human INSL3(115-131aa CCLSGCTQQDLLTLCPY), different from the related rat and mouse sequences by three amino acids.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Insulin-like 3(INSL3), a member of the insulin-like hormone superfamily, is specifically expressed in Leydig cells of the fetal and postnatal testis and in theca cells of the postnatal ovary. It is synthesized as a 131-amino acid preproprotein, which contains a 24-amino acid signal peptide. The human INSL3 gene is assigned to bands p13.2-p12 of the short arm of chromosome 19 with the similar organization to that of insulin and relaxin. INSL3 induces gubernaculum development in an androgen-independent way, while androgen-mediated regression of the CSL occurs independently from Insl3. Moreover, INSL3 is a ligand for LGR8 and INSL3-LGR8 mutations are believed to be associated with human cryptorchidism.

1. Adham IM, Burkhardt E, Benahmed M, Engel W.Cloning of a cDNA for a novel insulin-like peptide of the testicular Leydig cells. J Biol Chem. 1993 Dec 15; 268(35):26668-72. 2. Burkhardt E, Adham IM, Brosig B, Gastmann A, Mattei MG, Engel W.Structural organization of the porcine and human genes coding for a Leydig cell-specific insulin-like peptide (LEY I-L) and chromosomal localization of the human gene (INSL3). Genomics. 1994 Mar 1; 20(1):13-9. 3. Zimmermann S, Steding G, Emmen JM, Brinkmann AO, Nayernia K, Holstein AF, Engel W, Adham IM.Targeted disruption of the Insl3 gene causes bilateral cryptorchidism. Mol Endocrinol. 1999 May; 13(5):681-91. 4. Foresta C, Ferlin A.Role of INSL3 and LGR8 in cryptorchidism and testicular functions. Reprod Biomed Online. 2004 Sep; 9(3):294-8.

INSL3

Insulin-like 3

insulin-like 3(Leydig cell)

INSL 3 antibody INSL 3 antibody INSL3 antibody INSL3_HUMAN antibody Insulin like 3 antibody Insulin-like 3 A chain antibody Ley IL antibody Ley-I-L antibody Leydig insulin like peptide antibody Leydig insulin-like peptide antibody Relaxin like factor antibody Relaxin-like factor antibody RLF antibody RLNL antibody

P51460