Anti-HIF-1-alpha Antibody | Boster Immunoleader PA1041 product information

product summary

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-HIF-1-alpha Antibody

catalog :

PA1041

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

Boster Immunoleader product webpage

citations: 22

	Wang J, Jing C, Shan K, Chen Y, Guo X, Cao Z, et al. Semaphorin 4D and hypoxia-inducible factor-1α overexpression is related to prognosis in colorectal carcinoma. World J Gastroenterol. 2015;21:2191-8 pubmed publisher
	Weng T, Xie Y, Huang J, Luo F, Yi L, He Q, et al. Inactivation of Vhl in osteochondral progenitor cells causes high bone mass phenotype and protects against age-related bone loss in adult mice. J Bone Miner Res. 2014;29:820-9 pubmed publisher
	Weng T, Xie Y, Yi L, Huang J, Luo F, Du X, et al. Loss of Vhl in cartilage accelerated the progression of age-associated and surgically induced murine osteoarthritis. Osteoarthritis Cartilage. 2014;22:1197-205 pubmed publisher
	Wang T, Zhou Y, Chen X, Zhu A. Putative role of ischemic postconditioning in a rat model of limb ischemia and reperfusion: involvement of hypoxia-inducible factor-1α expression. Braz J Med Biol Res. 2014;47:738-45 pubmed
	Wang F, Liu S, Xi S, Yan L, Wang H, Song Y, et al. Arsenic induces the expressions of angiogenesis-related factors through PI3K and MAPK pathways in SV-HUC-1 human uroepithelial cells. Toxicol Lett. 2013;222:303-11 pubmed publisher
	He C, Sun X, Qiao H, Jiang X, Wang D, Jin X, et al. Downregulating hypoxia-inducible factor-2α improves the efficacy of doxorubicin in the treatment of hepatocellular carcinoma. Cancer Sci. 2012;103:528-34 pubmed publisher
	Wu H, Wang H, Zhang B, Zhang G, Zhang R, Zhang L. Puerarin decreases hypoxia inducible factor-1 alpha in the hippocampus of vascular dementia rats. Neural Regen Res. 2012;7:421-5 pubmed publisher
	Wang H, Wu H, Guo H, Zhang G, Zhang R, Zhan S. Increased hypoxia-inducible factor 1alpha expression in rat brain tissues in response to aging. Neural Regen Res. 2012;7:778-82 pubmed publisher
	Sun D, Wang Y, Liu C, Zhou X, Li X, Xiao A. Effects of nitric oxide on renal interstitial fibrosis in rats with unilateral ureteral obstruction. Life Sci. 2012;90:900-9 pubmed publisher
	Liu F, Tan G, Li J, Dong X, Krissansen G, Sun X. Gene transfer of endostatin enhances the efficacy of doxorubicin to suppress human hepatocellular carcinomas in mice. Cancer Sci. 2007;98:1381-7 pubmed
	Chen X, Wang S, Du J, Chen X. Diversities in hepatic HIF-1, IGF-I/IGFBP-1, LDH/ICD, and their mRNA expressions induced by CoCl(2) in Qinghai-Tibetan plateau mammals and sea level mice. Am J Physiol Regul Integr Comp Physiol. 2007;292:R516-26 pubmed
	Cao Y, Chen X, Wang S, Wang Y, Du J. Evolution and regulation of the downstream gene of hypoxia-inducible factor-1alpha in naked carp (Gymnocypris przewalskii) from Lake Qinghai, China. J Mol Evol. 2008;67:570-80 pubmed publisher
	Liu F, Wang P, Jiang X, Tan G, Qiao H, Jiang H, et al. Antisense hypoxia-inducible factor 1alpha gene therapy enhances the therapeutic efficacy of doxorubicin to combat hepatocellular carcinoma. Cancer Sci. 2008;99:2055-61 pubmed publisher
	Sun H, Qiu Z, Liu J, Sun J, Jiang T, Huang K, et al. Expression of hypoxia-inducible factor-1 alpha and associated proteins in pancreatic ductal adenocarcinoma and their impact on prognosis. Int J Oncol. 2007;30:1359-67 pubmed
	Mou K, Chen M, Mao Q, Wang P, Ni R, Xia X, et al. AQP-4 in peritumoral edematous tissue is correlated with the degree of glioma and with expression of VEGF and HIF-alpha. J Neurooncol. 2010;100:375-83 pubmed publisher
	Li Q, Dai A. Hypoxia inducible factor-1 alpha correlates the expression of heme oxygenase 1 gene in pulmonary arteries of rat with hypoxia-induced pulmonary hypertension. Acta Biochim Biophys Sin (Shanghai). 2004;36:133-40 pubmed
	Li Q, Dai A. Differential expression of three hypoxia-inducible factor-alpha subunits in pulmonary arteries of rat with hypoxia-induced hypertension. Acta Biochim Biophys Sin (Shanghai). 2005;37:665-72 pubmed
	Wu W, Pan C, Yu H, Gong H, Wang Y. Heparanase expression in gallbladder carcinoma and its correlation to prognosis. J Gastroenterol Hepatol. 2008;23:491-7 pubmed
	Ke J, Liu Y, Long X, Li J, Fang W, Meng Q, et al. Up-regulation of vascular endothelial growth factor in synovial fibroblasts from human temporomandibular joint by hypoxia. J Oral Pathol Med. 2007;36:290-6 pubmed
	Jiang Y, Dai A, Li Q, Hu R. Hypoxia induces transforming growth factor-beta1 gene expression in the pulmonary artery of rats via hypoxia-inducible factor-1alpha. Acta Biochim Biophys Sin (Shanghai). 2007;39:73-80 pubmed
	Wu W, Chen X, Hu C, Li J, Yu Z, Cai W. Transplantation of neural stem cells expressing hypoxia-inducible factor-1alpha (HIF-1alpha) improves behavioral recovery in a rat stroke model. J Clin Neurosci. 2010;17:92-5 pubmed publisher
	Zhang L, Li Q. Embryo-chondrocyte expressed gene 1, downregulating hypoxia-inducible factor 1alpha, is another marker of lung tumor hypoxia. Acta Pharmacol Sin. 2007;28:549-58 pubmed

product information

Product Name :

Anti-HIF-1-alpha Antibody

Catalog Number :

PA1041

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Hypoxia-inducible factor 1-alpha(HIF1A) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, rat Predicted to work with: mouse

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the N-terminus of human HIF-1-alpha(8-21aa NDKKKISSERRKEK), different from the related rat and mouse sequences by two amino acids.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

HIF-1 alpha(Hypoxia-inducible factor 1 alpha,HIF1A) is a transcription factor that mediates cellular and systemic homeostatic responses to reduced availability in mammals, including angiogenesis, erythropoiesis and glycolysis. This gene was mapped to 14q21-q24. HIF-1 alpha transactivate genes required for energy metabolism and tissue perfusion and is necessary for embryonic development and tumor explant growth. HIF-1 alpha is over expressed during carcinogenesis, myocardial infarction and wound healing. It is crucial for the cellular response to hypoxia and is frequently over expressed in human cancers, resulting in the activation of genes essential for cell survival. HIF-1 alpha regulates the survival and function in the inflammatory microenvironment directly. It is a transcription factor that plays a pivotal role in cellular adaptation to changes in oxygen availability.

Reference :

1. Sutter, C. H.; Laughner, E.; Semenza, G. L. : Hypoxia-inducible factor 1-alpha protein expression is controlled by oxygen-regulated ubiquitination that is disrupted by deletions and missense mutations. Proc. Nat. Acad. Sci. 97: 4748-4753, 2000. 2. Elson, D. A.; Thurston, G.; Huang, L. E.; Ginzinger, D. G.; McDonald, D. M.; Johnson, R. S.; Arbeit, J. M. : Induction of hypervascularity without leakage or inflammation in transgenic mice overexpressing hypoxia-inducible factor-1-alpha. Genes Dev. 15: 2520-2532, 2001. 3. Koshiji, M.; To, K. K.-W.; Hammer, S.; Kumamoto, K.; Harris, A. L.; Modrich, P.; Huang, L. E. : HIF-1-alpha induces genetic instability by transcriptionally downregulating MutS-alpha expression. Molec. Cell 17: 793-803, 2005. 4. Ivan, M.; Kondo, K.; Yang, H.; Kim, W.; Valiando, J.; Ohh, M.; Salic, A.; Asara, J. M.; Lane, W. S.; Kaelin, W. G., Jr. : HIF-alpha targeted for VHL-mediated destruction by proline hydroxylation: implications for O(2) sensing. Science 292: 464-468, 2001.

Gene Name :

HIF1A

Protein Name :

Hypoxia-inducible factor 1-alpha(HIF-1-alpha/HIF1-alpha)

Gene Full Name :

hypoxia inducible factor 1, alpha subunit(basic helix-loop-helix transcription factor)

Synonyms :

ARNT interacting protein antibody ARNT-interacting protein antibody Basic helix loop helix PAS protein MOP1 antibody Basic-helix-loop-helix-PAS protein MOP1 antibody bHLHe78 antibody Class E basic helix-loop-helix protein 78 antibody HIF 1A antibody HIF 1alpha antibody HIF-1-alpha antibody HIF1 A antibody HIF1 Alpha antibody HIF1 antibody HIF1-alpha antibody HIF1A antibody HIF1A_HUMAN antibody Hypoxia inducible factor 1 alpha antibody Hypoxia inducible factor 1 alpha isoform I.3 antibody Hypoxia inducible factor 1 alpha subunit antibody Hypoxia inducible factor 1 alpha subunit basic helix loop helix transcription factor antibody Hypoxia inducible factor 1, alpha subunit(basic helix loop helix transcription factor) antibody Hypoxia inducible factor1alpha antibody Hypoxia-inducible factor 1-alpha antibody Member of PAS protein 1 antibody Member of PAS superfamily 1 antibody Member of the PAS Superfamily 1 antibody MOP 1 antibody MOP1 antibody PAS domain-containing protein 8 antibody PASD 8 antibody PASD8 antibody

Uniprot ID :

Q16665

more info or order :

Boster Immunoleader product webpage