Anti-Connexin 43/GJA1 Antibody | Boster Immunoleader PA1026-1 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Connexin 43/GJA1 Antibody

catalog :

PA1026-1

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

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product information

Product Name :

Anti-Connexin 43/GJA1 Antibody

Catalog Number :

PA1026-1

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Gap junction alpha-1 protein(GJA1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, rat Predicted to work with: mouse

Applications :

WB,IHC-P,IHC-F,ICC

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Rat; Predicted Species: Human, Mouse

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Immunohistochemistry(Frozen Section) :

Concentration: 0.5-1μg/ml; Tested Species: Rat; Predicted Species: Human, Mouse

Immunocytochemistry :

Concentration: 0.5-1μg/ml; Tested Species: Human; Predicted Species: Mouse, Rat

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human Connexin 43(351-367aa HELQPLAIVDQRPSSRA), identical to the related rat and mouse sequences.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P), IHC(F) and ICC.

Background :

Connexins 43(Cx43), also called GAP Junction Protein, alpha-1(GJA1). Connexin 43 is a member of the connexin gene family which abundantly expressed in the heart and liver and was mapped to 6q21-q23.2. Connexin43, the major protein of gap junctions in the heart, is targeted by several protein kinases that regulate myocardial cell-cell coupling. Mutations in the connexin43 gap-junction gene, which lead to abnormally regulated cell-cell communication, are associated with visceroatrial heterotaxia. Cx43 must also play a critical role in the physiology of hearing, presumably by participating in the recycling of potassium to the cochlear endolymph.

Reference :

1. Britz-Cunningham, S. H.; Shah, M. M.; Zuppan, C. W.; Fletcher, W. H. : Mutations of the connexin43 gap-junction gene in patients with heart malformations and defects of laterality. New Eng. J. Med. 332: 1323-1329, 1995. 2. Liu, X. Z.; Xia, X. J.; Adams, J.; Chen, Z. Y.; Welch, K. O.; Tekin, M.; Ouyang, X. M.; Kristiansen, A.; Pandya, A.; Balkany, T.; Arnos, K. S.; Nance, W. E. : Mutations in GJA1 (connexin 43) are associated with non-syndromic autosomal recessive deafness. Hum. Molec. Genet. 10: 2945-2951, 2001.

Gene Name :

GJA1

Protein Name :

Gap junction alpha-1 protein

Gene Full Name :

gap junction protein, alpha 1, 43kDa

Synonyms :

Connexin 43 antibody Connexin-43 antibody Connexin43 antibody Cx 43 antibody CX43 antibody CXA1_HUMAN antibody DFNB38 antibody Gap junction 43 kDa heart protein antibody Gap junction alpha 1 protein antibody Gap junction alpha-1 protein antibody Gap junction protein alpha 1 43kD antibody Gap junction protein alpha 1 43kDa(connexin 43) antibody Gap junction protein alpha 1 43kDa antibody Gap junction protein alpha like antibody GJA 1 antibody Gja1 antibody GJAL antibody HGNC:4282 antibody HGNC:8112 antibody Oculodentodigital dysplasia antibody ODD antibody ODDD antibody ODOD antibody SDTY 3 antibody SDTY3 antibody Syndactyly type III antibody

Uniprot ID :

P17302

more info or order :

Boster Immunoleader product webpage