Boster Immunoleader

antibody

Anti-GJB2 Antibody

PA1025

100 ug/vial

200 USD

polyclonal

rabbit

human, mouse, rat

Anti-GJB2 Antibody

PA1025

Polyclonal

Rabbit IgG polyclonal antibody for Gap junction beta-2 protein(GJB2) detection. Tested with WB in Human;Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, rat Predicted to work with: mouse

WB

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the N-terminus of human GJB2(1-16aa, MDWGTLQTILGGVNKH), different from the related mouse and rat sequences by two amino acids.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Connexin26(CX26), also known as GAP junction protein, beta2, GJB2. Gap junctions were first characterized by electron microscopy as regionally specialized structures on plasma membranes of contacting adherent cells. These structures were shown to consist of cell-to-cell channels. Proteins, called connexins, purified from fractions of enriched gap junctions from different tissues differ. The 3-prime untranslated region of the CX26 transcript contains a putative mRNA instability sequence. The deduced 226-amino acid protein has a calculated molecular mass of about 26 kD. CX26 shares 92.5% identity with rat Cx26. connexin 26(GJB2) is assigned to human chromosome 13q11-q12 .Connexin 26 regulates epidermal barrier and wound remodeling and promotes psoriasiform response. Connexin 26 gene(GJB2) mutation modulates the severity of hearing loss associated with the 1555A-G mitochondrial mutation.

1. Mignon, C.; Fromaget, C.; Mattei, M.-G.; Gros, D.; Yamasaki, H.; Mesnil, M. : Assignment of connexin 26 (GJB2) and 46 (GJA3) genes to human chromosome 13q11-q12 and mouse chromosome 14D1-E1 by in situ hybridization. Cytogenet. Cell Genet. 72: 185-186, 1996. 2. Djalilian, A. R.; McGaughey, D.; Patel, S.; Seo, E. Y.; Yang, C.; Cheng, J.; Tomic, M.; Sinha, S.; Ishida-Yamamoto, A.; Segre, J. A. : Connexin 26 regulates epidermal barrier and wound remodeling and promotes psoriasiform response. J. Clin. Invest. 116: 1243-1253, 2006. 3. Abe, S.; Kelley, P. M.; Kimberling, W. J.; Usami, S. : Connexin 26 gene (GJB2) mutation modulates the severity of hearing loss associated with the 1555A-G mitochondrial mutation. Am. J. Med. Genet. 103: 334-338, 2001.

GJB2

Gap junction beta-2 protein

gap junction protein, beta 2, 26kDa

connexin 26 antibody Connexin-26 antibody cx26 antibody CXB2_HUMAN antibody DFNA3 antibody DFNB1 antibody Gap junction beta-2 protein antibody GJB2 antibody HID antibody KID antibody NSRD1 antibody PPK antibody

P29033