Anti-CD40L Antibody | Boster Immunoleader PA1020 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-CD40L Antibody

catalog :

PA1020

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human

application :

western blot, immunohistochemistry - paraffin section

publications citing this reagent :

Yang G, Fang Z, Liu Y, Zhang H, Shi X, Ji Q, et al. Protective effects of chinese traditional medicine buyang huanwu decoction on myocardial injury. Evid Based Complement Alternat Med. 2011;2011:930324 pubmed

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product information

Product Name :

Anti-CD40L Antibody

Catalog Number :

PA1020

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for CD40 ligand(CD40LG) detection. Tested with WB, IHC-P in Human.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the N-terminus of human CD40L(47-65aa HRRLDKIEDERNLHEDFVF), different from the related mouse sequence by three amino acids, rat sequence by four amino acids.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

CD40 ligand(CD40L) is a type II membrane protein of 261 amino acids on activated T cells that induces B cell proliferation and immunoglobulin secretion. It has homology with tumour necrosis factor-alpha and -beta, and has important functions in B-cell activation and differentiation. Human CD40L with 5 exons, is mapped to the proximal region of the mouse X chromosome on Xq26.3-27.1, and can be detected on T cells but is absent from B cells and monocytes. Since CD40L is expressed on platelets and released from them on activation, its predictive value as a marker for clinical outcome and the therapeutic effect of inhibition of glycoprotein IIb /IIIa receptor in patients with acute coronary syndromes was investigated. The soluble CD40L may be involved in the process of restenosis and that it exerts its effect by triggering a complex group of inflammatory reactions on endothelial and mononuclear cells. CD40L plays a central role in the pathophysiology of acute coronary syndromes, and has a role in the pathogenesis of coronary artery lesions.

Reference :

1. Allen,R.C.;Armitage,R.J.;Conley,M.E.;Rosenblatt,H.;Jenkins,N.A.;Copeland,N.G.;Bedell, M.A.;Edelhoff,S.;Disteche,C.M.;Simoneaux,D.K.;Fanslow,W.C.;Belmont,J.;Spriggs,M.K.:CD40 ligand gene defects responsible for X-linked hyper-IgM syndrome. Science 259: 990-993, 1993. 2. Cipollone,F.;Ferri,C.;Desideri,G.;Paloscia,L.;Materazzo,G.;Mascellanti,M.;Fazia,M.; Iezzi,A.;Cuccurullo,C.;Pini,B.;Bucci,M.;Santucci,A.;Cuccurullo,F.;Mezzetti,A.:Preprocedural level of soluble CD40L is predictive of enhanced inflammatory response and restenosis after coronary angioplasty. Circulation 108: 2776-2782, 2003.

Gene Name :

CD40LG

Protein Name :

CD40 ligand(CD40-L)

Gene Full Name :

CD40 ligand

Synonyms :

CD 154 antibody CD 40L antibody CD154 antibody CD154 antigen antibody CD40 antigen ligand antibody CD40 homologue antibody CD40 L antibody CD40 ligand antibody CD40 ligand, soluble form antibody CD40-L antibody CD40L_HUMAN antibody CD40LG antibody gp39 antibody hCD40L antibody HIGM1 antibody Hyper IgM syndrome antibody IGM antibody IMD3 antibody Ly62 antibody T B cell activating molecule antibody T BAM antibody T cell antigen GP39 antibody T-cell antigen Gp39 antibody TBAM antibody TNF related activation protein antibody TNF superfamily member 5 antibody TNF-related activation protein antibody Tnfsf5 antibody TRAP antibody Tumor Necrosis Factor(ligand) Superfamily Member 5 antibody Tumor necrosis factor ligand superfamily member 5 antibody

Uniprot ID :

P29965

more info or order :

Boster Immunoleader product webpage