Boster Immunoleader

antibody

Anti-CD22 Antibody

PA1018-1

100 ug/vial

200 USD

polyclonal

rabbit

western blot, immunohistochemistry - paraffin section

Anti-CD22 Antibody

PA1018-1

Polyclonal

Rabbit IgG polyclonal antibody for B-cell receptor CD22(CD22) detection. Tested with WB, IHC-P in Human.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Concentration: 0.5-1μg/ml; Tested Species: Human

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the C-terminus of human CD22(832-847aa ERPQAQENVDYVILKH).

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

CD22 is a surface glycoprotein of B lymphocytes that is rapidly phosphorylated on cytoplasmic tyrosines after antigen receptor cross-linking. CD22 is a negative regulator of antigen receptor signaling whose onset of expression at the mature B cell stage may serve to raise the antigen concentration threshold required for B cell triggering. The human CD22 gene is expressed specifically in B lymphocytes and likely has an important function in cell-cell interactions. The B cell coreceptor CD22 plays an important role in regulating signal transduction via the B cell Ag receptor.3 CD22 is located within the band region q13.1 of chromosome 19.

1. O'Keefe, T. L.; Williams, G. T.; Davies, S. L.; Neuberger, M. S. Hyperresponsive B cells in CD22-deficient mice. Science 274: 798-801, 1996. 2. Wilson, G. L.; Najfeld, V.; Kozlow, E.; Menniger, J.; Ward, D.; Kehrl, J. H. Genomic structure and chromosomal mapping of the human CD22 gene. J. Immun. 150: 5013-5024, 1993. 3. John, B.; Herrin, B. R.; Raman, C.; Wang, Y.; Bobbitt, K. R.; Brody, B. A.; Justement, L. B. The B cell coreceptor CD22 associates with AP50, a clathrin-coated pit adapter protein, via tyrosine-dependent interaction. J. Immun. 170: 3534-3543, 2003.

CD22

B-cell receptor CD22

CD22 molecule

B cell receptor CD22 precursor antibody B lymphocyte cell adhesion molecule antibody B-cell receptor CD22 antibody B-lymphocyte cell adhesion molecule antibody BL CAM antibody BL-CAM antibody BLCAM antibody CD 22 antibody CD22 antibody CD22 antigen antibody CD22 molecule antibody CD22 protein antibody CD22_HUMAN antibody Lectin 2 antibody Leu14 antibody Lyb8 antibody MGC130020 antibody sialic acid binding Ig like lectin 2 antibody Sialic acid binding immunoglobulin like lectin 2 antibody Sialic acid-binding Ig-like lectin 2 antibody SIGLEC 2 antibody Siglec-2 antibody SIGLEC2 antibody T cell surface antigen Leu 14 antibody T-cell surface antigen Leu-14 antibody

P20273