Anti-ATP5J Antibody | Boster Immunoleader PA1012 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-ATP5J Antibody

catalog :

PA1012

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-ATP5J Antibody

Catalog Number :

PA1012

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for ATP synthase-coupling factor 6, mitochondrial(ATP5J) detection. Tested with WB in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, rat Predicted to work with: mouse

Applications :

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence in the middle region of human ATP5J(CF6), different from the related mouse sequence by two amino acids.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Background :

ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F6(ATP5J) is a multisubunit membrane-bound enzyme complex consisting of an F0 segment embedded in the membrane and an F1 segment attached to the F0. It is also a component of mitochondrial ATP synthase which is required for the interactions of the catalytic and proton-translocating segments. Human ATP5J shares 72% sequence identity with rat ATP5J. This import signal peptide is rich in basic amino acids, devoid of acidic amino acids, and amphiphilic, which allows it to be water-soluble yet capable of passage through the phospholipid membrane bilayers. Moreover, it is circulating and functions as an endogenous vasoconstrictor by inhibiting cytosolic phospholipase A2.

Reference :

1. Javed AA, Ogata K, Sanadi DR. Human mitochondrial ATP synthase: cloning cDNA for the nuclear-encoded precursor of coupling factor 6. Gene. 1991 Jan 15; 97(2):307-10. 2. Higuti T, Tsurumi C, Kawamura Y, Tsujita H, Osaka F, Yoshihara Y, Tani I, Tanaka K, Ichihara A. Molecular cloning of cDNA for the import precursor of human coupling factor 6 of H(+)-ATP synthase in mitochondria. Biochem Biophys Res Commun. 1991 Jul 31; 178(2):793-9. 3. Osanai T, Magota K, Tanaka M, Shimada M, Murakami R, Sasaki S, Tomita H, Maeda N, Okumura K. Intracellular signaling for vasoconstrictor coupling factor 6: novel function of beta-subunit of ATP synthase as receptor. Hypertension. 2005 Nov; 46(5):1140-6.

Gene Name :

ATP5J

Protein Name :

ATP synthase-coupling factor 6, mitochondrial(ATPase subunit F6)

Gene Full Name :

ATP synthase, H+ transporting, mitochondrial Fo complex, subunit F6

Synonyms :

ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F6 antibody ATP synthase-coupling factor 6, mitochondrial antibody ATP5 antibody ATP5A antibody ATP5J antibody ATP5J_HUMAN antibody ATPase subunit F6 antibody ATPM antibody CF6 antibody F6 antibody

Uniprot ID :

P18859

more info or order :

Boster Immunoleader product webpage