product summary
request information :
company name :
Boster Immunoleader
product type :
antibody
product name :
Anti-Annexin V Antibody
catalog :
PA1008
quantity :
100 ug/vial
price :
200 USD
clonality :
polyclonal
host :
rabbit
reactivity :
human, mouse, rat
application :
western blot, immunocytochemistry, immunohistochemistry - paraffin section
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product information
Product Name :
Anti-Annexin V Antibody
Catalog Number :
PA1008
Clonality :
Polyclonal
Description short :
Rabbit IgG polyclonal antibody for Annexin A5(ANXA5) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.
Price :
200 USD
Ig type :
Rabbit IgG
Size :
100 ug/vial
Form :
Lyophilized
Specificity :
No cross reactivity with other proteins.
Reactivity :
Reacts with: human, rat
Predicted to work with: mouse
Applications :
WB,IHC-P,ICC
Western blot :
Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse
Immunohistochemistry(Paraffin-embedded S ... :
Concentration: 0.5-1μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse
Immunocytochemistry :
Concentration: 0.5-1μg/ml; Tested Species: Human
Notes :
Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
Immunogen :
A synthetic peptide corresponding to a sequence in the middle region of human Annexin V(88-102aa SRLYDAYELKHALKG), identical to the related rat and mouse sequences.
Contents :
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Purification :
Immunogen affinity purified.
Reconstitution :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage :
At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.
Relevant detection systems :
Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P) and ICC.
Background :
Annexin V also know as endonnexin II(ENX2),or placental protein 4(PP4). Endonexin II is a member of the family of Ca(2+)-dependent phospholipid binding proteins, known as annexins. It bind to the phospholipids that are preferentially located on the cytosolic face of the plasma membrane. It has a relative molecular weight of about 35,000. The gene lies on mouse chromosome 3 in close linkage with the fibroblast growth factor 2(basic) gene and is syntenic with other genes known to have orthologous counterparts on human chromosome 4q. The PP4 cDNA encoded a protein of 320 amino acid residues. A single mRNA, approximately 1.6 kb long, was found to be expressed in human cell lines and placenta. PP4 is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
Reference :
1. Kaplan, R.; Jaye, M.; Burgess, W. H.; Schlaepfer, D. D.; Haigler, H. T. : Cloning and expression of cDNA for human endonexin II, a Ca(2+) and phospholipid binding protein. J. Biol. Chem. 263
2. Rodriguez-Garcia, M. I.; Kozak, C. A.; Morgan, R. O.; Fernandez, M. P. : Mouse annexin V chromosomal localization, cDNA sequence conservation, and molecular evolution. Genomics 31: 151-157, 1996.
3. Grundmann, U.; Abel, K.-J.; Bohn, H.; Lobermann, H.; Lottspeich, F.; Kupper, H. : Characterization of cDNA encoding human placental anticoagulant protein (PP4): homology with the lipocortin family. Proc. Nat. Acad. Sci. 85: 3708-3712, 1988.
Gene Name :
ANXA5
Protein Name :
Annexin A5
Gene Full Name :
annexin A5
Synonyms :
Anchorin CII antibody Annexin 5 antibody Annexin A5 antibody Annexin V antibody Annexin-5 antibody Annexin5 antibody AnnexinA5 antibody AnnexinV antibody ANX 5 antibody ANX A5 antibody ANX5 antibody ANXA5 antibody ANXA5_HUMAN antibody Calphobindin I antibody CBP I antibody CBP-I antibody Endonexin II antibody ENX 2 antibody ENX2 antibody Lipocortin V antibody PAP I antibody PAP-I antibody Placental anticoagulant protein 4 antibody Placental anticoagulant protein I antibody PLACENTAL PROTEIN 4 antibody PP 4 antibody PP4 antibody Thromboplastin inhibitor antibody VAC alpha antibody VAC-alpha antibody Vascular anticoagulant alpha antibody Vascular anticoagulant-alpha antibody
Uniprot ID :
P08758
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company information

Boster Immunoleader
3942 B Valley Ave
Pleasanton, CA 94566
Pleasanton, CA 94566
boster@bosterbio.com
www.bosterbio.com925.485.4527
headquarters: USA
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