Anti-Fibronectin Antibody (monoclonal) | Boster Immunoleader MA1116 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Fibronectin Antibody (monoclonal)

catalog :

MA1116

quantity :

100 ug/vial

price :

200 USD

clonality :

monoclonal

host :

mouse

reactivity :

human, mouse, rat, chicken

application :

western blot, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

publications citing this reagent :

Zhou Y, Lv C, Wu C, Chen F, Shao Y, Wang Q. Suppressor of cytokine signaling (SOCS) 2 attenuates renal lesions in rats with diabetic nephropathy. Acta Histochem. 2014;116:981-8 pubmed publisher
Yu X, Li C, Li X, Cai L. Rosiglitazone prevents advanced glycation end products-induced renal toxicity likely through suppression of plasminogen activator inhibitor-1. Toxicol Sci. 2007;96:346-56 pubmed

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Fibronectin Antibody (monoclonal)

Catalog Number :

MA1116

Clonality :

Monoclonal (Clone: FN3E2)

Description short :

Mouse IgG monoclonal antibody for Fibronectin, fibronectin 1 (FN1 ) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;mouse;rat;chicken. No cross reactivity with other proteins.

Price :

200 USD

Ig type :

Mouse IgG1

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Human, chicken, mouse.

Applications :

WB,IHC-P,IHC-F,ICC

Western blot :

At 1-2μg/ml with the appropriate system to detect Fibronectin in cells and tissues.

Immunohistochemistry(Paraffin-embedded S ... :

At 1-2μg/ml to detect Fibronectin in formalin fixed and paraffin embedded tissues.

Immunohistochemistry(Frozen Section) :

At 1-2μg/ml to detect Fibronectin in formalin/acetone fixed tissues.

Immunocytochemistry :

Suitable

Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

Fibronectin from human plasma.

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Reconstitution :

1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1001 in WB, supported by SA1021 in IHC(P), IHC(F) and ICC.

Background :

Fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. Cellular fibronectin is a 550KDa glycoprotein existing as two heterodimers, different from plasma fibronectin in the presence of additional polypeptide segments and in altering morphology of transformed cells and hemaglutination.

Reference :

1. Saad S, Gottieb DJ, Bradstock KF, Overall CM, Bendall Lj. Cancer cell-associated fibronectin induces release of matrix metalloproteinase-2 from normal fibroblasts. Cancer Res. 2002 Jan 1;62(1):283-9. 2. Saito N, Mitsuhashi M, Hayashi T, Narumo C, Nagata H, Soyama K, Kameoka S, Harumiya S, Fujimoto D. Inhibition of hepatic metastasis in mice treated with cell-binding domain of human fibronectin and angiogenesis inhibitor TNP-470. Int J Clin Oncol.2001 Oct;6(5):215-20. 3. Chassagne C, Adamy C, Ratajczak P, Gingras B, Teiger E, Planus E, Oliviero P, Pappaport L, Samuel JL, Meloche S. Angiotensin II AT(2) receptor inhibits smooth muscle cell migration via fibronectin cell production and binding. Am J Physiol Cell Physiol. 2002 Apr;282(4):C654-64.

Gene Name :

FN1

Protein Name :

Fibronectin

Gene Full Name :

fibronectin 1

Synonyms :

FN CIG FNZ MSF ED-B FINC GFND LETS GFND2

Uniprot ID :

Human(P02751), Mouse(P11276), Rat(P04937)

more info or order :

Boster Immunoleader product webpage