product summary
company name :
Boster Immunoleader
product type :
antibody
product name :
Anti-beta-Actin Antibody (monoclonal)
catalog :
MA1115
quantity :
100 ug/vial
price :
200 USD
clonality :
monoclonal
host :
mouse
clone name :
AC-15

The same clone is also sold as:
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry - paraffin section
more info or order :
Boster Immunoleader product webpage
citations: 34
Published Application/Species/DilutionReference
Wu C, Wu Q, Du J, Zeng J, Li T, Xu C, et al. Calcium-sensing receptor in the T lymphocyte enhanced the apoptosis and cytokine secretion in sepsis. Mol Immunol. 2015;63:337-42 pubmed publisher
Ma Y, Bu J, Dang H, Sha J, Jing Y, Shan-Jiang A, et al. Inhibition of adenosine monophosphate-activated protein kinase reduces glial cell-mediated inflammation and induces the expression of Cx43 in astroglias after cerebral ischemia. Brain Res. 2015;1605:1-11 pubmed publisher
Wang L, Gai P, Xu R, Zheng Y, Lv S, Li Y, et al. Shikonin protects chondrocytes from interleukin-1beta-induced apoptosis by regulating PI3K/Akt signaling pathway. Int J Clin Exp Pathol. 2015;8:298-308 pubmed
Zhao L, Li W, Zhou Y, Zhang Y, Huang S, Xu X, et al. The overexpression and nuclear translocation of Trx-1 during hypoxia confers on HepG2 cells resistance to DDP, and GL-V9 reverses the resistance by suppressing the Trx-1/Ref-1 axis. Free Radic Biol Med. 2015;82:29-41 pubmed publisher
Cheng C, Huang C, Ma T, Bian E, He Y, Zhang L, et al. SOCS1 hypermethylation mediated by DNMT1 is associated with lipopolysaccharide-induced inflammatory cytokines in macrophages. Toxicol Lett. 2014;225:488-97 pubmed publisher
Qi B, Chen H, Shang D, Dong Y, Zhang G, Yu L. Effects of hypoxia-inducible factor-1α and matrix metalloproteinase-9 on alveolar-capillary barrier disruption and lung edema in rat models of severe acute pancreatitis-associated lung injury. Exp Ther Med. 2014;8:899-906 pubmed
Wang T, Zhou Y, Chen X, Zhu A. Putative role of ischemic postconditioning in a rat model of limb ischemia and reperfusion: involvement of hypoxia-inducible factor-1α expression. Braz J Med Biol Res. 2014;47:738-45 pubmed
Shi J, Li J, Hu R, Shi Y, Su X, Guo X, et al. Tristetraprolin is involved in the glucocorticoid-mediated interleukin 8 repression. Int Immunopharmacol. 2014;22:480-5 pubmed publisher
Shen T, Liu K, Miao D, Cao R, Chen P. Effective inhibition of Japanese encephalitis virus replication by shRNAs targeting various viral genes in vitro and in vivo. Virology. 2014;454:48-59 pubmed publisher
Mana L, Wang S, Zhu H, Xing Y, Lou L, Wu A, et al. Qingkailing Suppresses the Activation of BV2 Microglial Cells by Inhibiting Hypoxia/Reoxygenation-Induced Inflammatory Responses. Evid Based Complement Alternat Med. 2014;2014:696218 pubmed publisher
Zhao M, Chen Y, Chen X, Dong X, Zhou J, Wang H, et al. Folic acid supplementation during pregnancy protects against lipopolysaccharide-induced neural tube defects in mice. Toxicol Lett. 2014;224:201-8 pubmed publisher
Yao J, Hu R, Sun J, Lin B, Zhao L, Sha Y, et al. Oroxylin A prevents inflammation-related tumor through down-regulation of inflammatory gene expression by inhibiting NF-κB signaling. Mol Carcinog. 2014;53:145-58 pubmed publisher
Li J, Zha X, Wang R, Li X, Xu B, Xu Y, et al. Regulation of CD44 expression by tumor necrosis factor-α and its potential role in breast cancer cell migration. Biomed Pharmacother. 2012;66:144-50 pubmed publisher
Meng L, Yu B. Oxygen- and glucose-deprived culture promotes cell proliferation and invasion of vascular smooth muscle cells. Int J Mol Med. 2011;28:777-83 pubmed publisher
Han M, Liu M, Wang Y, Chen X, Xu J, Sun Y, et al. Antagonism of miR-21 reverses epithelial-mesenchymal transition and cancer stem cell phenotype through AKT/ERK1/2 inactivation by targeting PTEN. PLoS ONE. 2012;7:e39520 pubmed publisher
Tang H, Deng M, Tang Y, Xie X, Guo J, Kong Y, et al. miR-200b and miR-200c as prognostic factors and mediators of gastric cancer cell progression. Clin Cancer Res. 2013;19:5602-12 pubmed publisher
Gao K, Li X, Zhang L, Bai L, Dong W, Gao K, et al. Transgenic expression of IL-33 activates CD8(+) T cells and NK cells and inhibits tumor growth and metastasis in mice. Cancer Lett. 2013;335:463-71 pubmed publisher
Kong J, Kong J, Pan B, Ke S, Dong S, Li X, et al. Insufficient radiofrequency ablation promotes angiogenesis of residual hepatocellular carcinoma via HIF-1α/VEGFA. PLoS ONE. 2012;7:e37266 pubmed publisher
Su Y, Liu W, Ma L, Liu X, Liu Z, Zhu B. Scutellarin inhibits translocation of protein kinase C in diabetic thoracic aorta of the rat. Clin Exp Pharmacol Physiol. 2012;39:136-40 pubmed publisher
Ni Y, Jiang T, Cheng Q, Gu Z, Zhu Y, Zhang Z, et al. Protective effect of magnolol on lipopolysaccharide-induced acute lung injury in mice. Inflammation. 2012;35:1860-6 pubmed publisher
Lang Q, Liu Q, Xu N, Qian K, Qi J, Sun Y, et al. The antifibrotic effects of TGF-β1 siRNA on hepatic fibrosis in rats. Biochem Biophys Res Commun. 2011;409:448-53 pubmed publisher
Wang Y, Tian F, Liu H, Zhang W, Li J, Xiao B, et al. Macrophage migration inhibitory factor is necessary for the lipo-oligosaccharide-induced response by modulation of Toll-like receptor 4 in monocytes from GBS patients. J Neuroimmunol. 2013;257:67-75 pubmed publisher
Chang Y, Wei W, Zhang L, Xu H. Effects and mechanisms of total glucosides of paeony on synoviocytes activities in rat collagen-induced arthritis. J Ethnopharmacol. 2009;121:43-8 pubmed publisher
Tang S, Wang X, Hong H, Long Y, Li Y, Xiang G, et al. Leukotriene D4 induces cognitive impairment through enhancement of CysLT₁ R-mediated amyloid-β generation in mice. Neuropharmacology. 2013;65:182-92 pubmed publisher
Zhang L, Wu S, Ruan Y, Hong L, Xing X, Lai W. Testosterone therapy delays cardiomyocyte aging via an androgen receptor-independent pathway. Braz J Med Biol Res. 2011;44:1118-24 pubmed
Wang S, Wu X, Chen Y, Zhang J, Ding J, Zhou Y, et al. Prognostic and predictive role of JWA and XRCC1 expressions in gastric cancer. Clin Cancer Res. 2012;18:2987-96 pubmed publisher
Bi Y, Sun W, Chen X, Li M, Liang H, Cai M, et al. Effect of early insulin therapy on nuclear factor kappaB and cytokine gene expressions in the liver and skeletal muscle of high-fat diet, streptozotocin-treated diabetic rats. Acta Diabetol. 2008;45:167-78 pubmed publisher
Ni Y, Wang J, Yan X, Tian F, Zhao J, Wang Y, et al. Histone deacetylase inhibitor, butyrate, attenuates lipopolysaccharide-induced acute lung injury in mice. Respir Res. 2010;11:33 pubmed publisher
Ni Y, Kuai J, Lu Z, Yang G, Fu H, Wang J, et al. Glycyrrhizin treatment is associated with attenuation of lipopolysaccharide-induced acute lung injury by inhibiting cyclooxygenase-2 and inducible nitric oxide synthase expression. J Surg Res. 2011;165:e29-35 pubmed publisher
Chen W, Fu X, Ge S, Sun T, Zhou G, Han B, et al. Profiling of genes differentially expressed in a rat of early and later gestational ages with high-density oligonucleotide DNA array. Wound Repair Regen. 2007;15:147-55 pubmed
Zhao M, Chen Y, Dong X, Zhou J, Chen X, Wang H, et al. Folic acid protects against lipopolysaccharide-induced preterm delivery and intrauterine growth restriction through its anti-inflammatory effect in mice. PLoS ONE. 2013;8:e82713 pubmed publisher
Su H, Yang J, Xu T, Huang J, Xu L, Yuan Y, et al. MicroRNA-101, down-regulated in hepatocellular carcinoma, promotes apoptosis and suppresses tumorigenicity. Cancer Res. 2009;69:1135-42 pubmed publisher
Chen Y, Zhao M, Chen X, Zhang Y, Wang H, Huang Y, et al. Zinc supplementation during pregnancy protects against lipopolysaccharide-induced fetal growth restriction and demise through its anti-inflammatory effect. J Immunol. 2012;189:454-63 pubmed publisher
Zhou H, Chen X, Zhang W, Zhu L, Cheng L. HIF-1α inhibition reduces nasal inflammation in a murine allergic rhinitis model. PLoS ONE. 2012;7:e48618 pubmed publisher
product information
Product Name :
Anti-beta-Actin Antibody (monoclonal)
Catalog Number :
MA1115
Clonality :
Monoclonal (Clone: AC-15)
Description short :
Mouse IgG monoclonal antibody for beta-Actin, actin, beta (ACTB) detection. Tested with WB, IHC-P in Human;mouse;rat. No cross reactivity with other proteins.
Price :
200 USD
Ig type :
Mouse IgG1
Size :
100 ug/vial
Form :
Lyophilized
Specificity :
No cross reactivity with other proteins.
Reactivity :
Human, mouse, rat
Applications :
WB,IHC-P
Western blot :
At 0.25-0.5μg/ml with the appropriate system to detect β-actin in cells and tissues.
Immunohistochemistry(Paraffin-embedded S ... :
At 0.5-1μg/ml to detect β-actin in formalin fixed and paraffin embedded tissues.
Notes :
Other applications have not been tested. Optimal dilutions should be determined by end users.
Immunogen :
slightly modified beta-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.
Contents :
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Purification :
Ascites
Reconstitution :
1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.
Storage :
At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.
Relevant detection systems :
Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1001 in WB, supported by SA1021 in IHC(P).
Background :
The primary site of action of cytochalasin B on cell motility processes is beta-actin. Habets et al.(1992) generated hybrids that harbor only specific regions of human chromosome 7 and assigned the ACTB locus to 7p15-p12. ACTB and the other assigned beta-actin-related sequences are dispersed over at least four different chromosomes including one locus assigned to the X chromosome. A mutation of beta-actin that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia.
Reference :
1. Ng, S.-Y.; Gunning, P.; Eddy, R.; Ponte, P.; Leavitt, J.; Kedes, L.; Shows, T. : Chromosome 7 assignment of the human beta-actin functional gene (ACTB) and the chromosomal dispersion of pseudogenes. (Abstract) Cytogenet. Cell Genet. 40: 712 only, 1985. 2. Procaccio, V.; Salazar, G.; Ono, S.; Styers, M. L.; Gearing, M.; Davila, A.; Jimenez, R.; Juncos, J.; Gutekunst, C.-A.; Meroni, G.; Fontanella, B.; Sontag, E.; Sontag, J. M.; Faundez, V.; Wainer, B. H. : A mutation of beta-actin that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia. Am. J. Hum. Genet. 78: 947-960, 2006. 3. Ng, S.-Y.; Gunning, P.; Eddy, R.; Ponte, P.; Leavitt, J.; Shows, T.; Kedes, L. : Evolution of the functional human beta-actin gene and its multi-pseudogene family: conservation of the noncoding regions and chromosomal dispersion of pseudogenes. Molec. Cell. Biol. 5: 2720-2732, 1985.
Gene Name :
ACTB
Protein Name :
Actin, cytoplasmic 1
Gene Full Name :
actin, beta
Synonyms :
BRWS1 PS1TP5BP1 BETA-ACTIN ACTIN, CYTOPLASMIC, 1
Uniprot ID :
Human(P60709), Mouse(P60710), Rat(P60711)
more info or order :
Boster Immunoleader product webpage
company information
Boster Immunoleader
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
www.bosterbio.com
925.485.4527
headquarters: USA
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