Anti-gamma-Tubulin Antibody (monoclonal) | Boster Immunoleader MA1114 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-gamma-Tubulin Antibody (monoclonal)

catalog :

MA1114

quantity :

100 ug/vial

price :

200 USD

clonality :

monoclonal

host :

mouse

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-gamma-Tubulin Antibody (monoclonal)

Catalog Number :

MA1114

Clonality :

Monoclonal (Clone: IMD-20)

Description short :

Mouse IgG monoclonal antibody for gamma-Tubulin, golgi-associated, gamma adaptin ear containing, ARF binding protein 1; golgi-associated, gamma adaptin ear containing, ARF binding protein 2; golgi-associated, gamma adaptin ear containing, ARF binding protein 3 (GGA1; GGA2; GGA3 ) detection. Tested with WB in Human,rat. No cross reactivity with other proteins.

Price :

200 USD

Ig type :

Mouse IgG1

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Human, mouse, rat

Applications :

WB,ICC

Western blot :

At 0.25-0.5μg/ml with the appropriate system to detect γ-Tubulin in cells and tissues.

Immunocytochemistry :

Suitable

Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

Synthetic gamma-tubulin peptide(amino acids 38-53), conjugated to KLH.

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Reconstitution :

1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1001 in WB, supported by SA1021 in ICC.

Background :

Gamma-tubulin is a universal component of microtubule organizing centers, which is essential for nuclear division and microtubule assembly in Aspergillus nidulans. TUBG1 mapped to within 20 kb of TUBG2 at 17q21. Gamma-tubulin is a minor protein, present at less than 1% the level of alpha- and beta-tubulin, and is limited to the centrosome. Gamma-tubulin is present in Drosophila melanogaster and Homo sapiens.

Reference :

1. Rommens, J. M.; Durocher, F.; McArthur, J.; Tonin, P.; LeBlanc, J.-F.; Allen, T.; Samson, C.; Ferri, L.; Narod, S.; Morgan, K.; Simard, J. : Generation of a transcription map at the HSD17B locus centromeric to BRCA1 at 17q21. Genomics 28: 530-542, 1995. 2. Zheng, Y.; Jung, M. K.; Oakley, B. R. : Gamma-tubulin is present in Drosophila melanogaster and Homo sapiens and is associated with the centrosome. Cell 65: 817-823, 1991. 3. Stearns, T.; Evans, L.; Kirschner, M. : Gamma-tubulin is a highly conserved component of the centrosome. Cell 65: 825-836, 1991.

Gene Name :

GGA1; GGA2; GGA3

Protein Name :

ADP-ribosylation factor-binding protein GGA1; ADP-ribosylation factor-binding protein GGA2; ADP-ribosylation factor-binding protein GGA3

Gene Full Name :

golgi-associated, gamma adaptin ear containing, ARF binding protein 1; golgi-associated, gamma adaptin ear containing, ARF binding protein 2; golgi-associated, gamma adaptin ear containing, ARF binding protein 3

Synonyms :

VEAR KIAA1080 KIAA0154 Gamma-adaptin-related protein 1 Gamma-adaptin-related protein 2 Golgi-localized, gamma ear-containing, ARF-binding protein 2 VHS domain and ear domain of gamma-adaptin

Uniprot ID :

Human(Q9UJY5), Mouse(Q8R0H9), Rat(Q5FVF3), Human(Q9UJY4), Mouse(Q6P5E6), Rat(Q4G038), Human(Q9NZ52), Mouse(Q8BMI3), Rat(D3ZHG8)

more info or order :

Boster Immunoleader product webpage