Anti-beta-Tubulin Antibody (monoclonal) | Boster Immunoleader MA1112 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-beta-Tubulin Antibody (monoclonal)

catalog :

MA1112

quantity :

100 ug/vial

price :

200 USD

clonality :

monoclonal

host :

mouse

application :

western blot, immunohistochemistry - paraffin section

publications citing this reagent :

Cao S, Li S, Li H, Xiong L, Zhou Y, Fan J, et al. The potential role of HMGB1 release in peritoneal dialysis-related peritonitis. PLoS ONE. 2013;8:e54647 pubmed publisher
Ye Y, Zeng Y, Wan M, Lu X. Induction of human bone marrow mesenchymal stem cells differentiation into neural-like cells using cerebrospinal fluid. Cell Biochem Biophys. 2011;59:179-84 pubmed publisher

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-beta-Tubulin Antibody (monoclonal)

Catalog Number :

MA1112

Clonality :

Monoclonal (Clone: TUB-2)

Description short :

Mouse IgG monoclonal antibody for beta-Tubulin detection. Tested with WB, IHC-P in Human,rat. No cross reactivity with other proteins.

Price :

200 USD

Ig type :

Mouse IgG1

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Human, mouse, rat

Applications :

WB,IHC-P

Western blot :

At 2μg/ml with the appropriate system to detect β-Tubulin in cells and tissues.

Immunohistochemistry(Paraffin-embedded S ... :

At 0.5-1μg/ml to detect β-Tubulin in formalin fixed and paraffin embedded tissues.

Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

Tubulin from rat brain.

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Reconstitution :

1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1001 in WB, supported by SA1021 in IHC(P).

Background :

Tubulin beta(TUBB) was derived from its corresponding mRNA with a molecular weight of about 55,000. TUBB gene contains 4 exons and located in the HLA class I region at 6p21.3. Paclitaxel resistance in non-small-cell lung cancer associated with beta-tubulin gene mutations.

Reference :

1. Floyd-Smith, G. A.; de Martinville, B.; Francke, U. : A beta-tubulin expressed gene M40 (TUBB) is located on human chromosome 6 and two related pseudogenes are located on chromosomes 8 (TUBBP1) and 13 (TUBBP2). (Abstract) Cytogenet. Cell Genet. 40: 629, 1985. 2. Floyd-Smith, G. A.; de Martinville, B.; Francke, U. : An expressed beta-tubulin gene, TUBB, is located on the short arm of human chromosome 6 and two related sequences are dispersed on chromosomes 8 and 13. Exp. Cell Res. 163: 539-548, 1986.

Uniprot ID :

Human(Q9H4B7), Mouse(A2AQ07), Rat(M0R8B6), Human(Q13885), Mouse(Q7TMM9), Rat(P85108), Human(Q9BVA1), Mouse(Q9CWF2), Rat(Q3KRE8), Human(Q13509), Mouse(Q9ERD7), Rat(Q4QRB4), Human(P04350), Mouse(Q9D6F9), Rat(B4F7C2), Human(P68371), Mouse(P68372), Rat(Q6P9T8), Human(P07437), Mouse(P99024), Rat(P69897)

more info or order :

Boster Immunoleader product webpage