Anti-alpha-Tubulin Antibody (monoclonal) | Boster Immunoleader MA1107 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-alpha-Tubulin Antibody (monoclonal)

catalog :

MA1107

quantity :

100 ug/vial

price :

200 USD

clonality :

monoclonal

host :

mouse

application :

western blot, immunohistochemistry - paraffin section

publications citing this reagent :

Shen L, Liu X, Hou W, Yang G, Wu Y, Zhang R, et al. NDRG2 is highly expressed in pancreatic beta cells and involved in protection against lipotoxicity. Cell Mol Life Sci. 2010;67:1371-81 pubmed publisher

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-alpha-Tubulin Antibody (monoclonal)

Catalog Number :

MA1107

Clonality :

Monoclonal (Clone: Tub-1)

Description short :

Mouse IgG monoclonal antibody for alpha-Tubulin detection. Tested with WB, IHC-P in Human;rat. No cross reactivity with other proteins.

Price :

200 USD

Ig type :

Mouse IgG1

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Human, mouse, rat, chicken

Applications :

WB,IHC-P

Western blot :

At 2μg/ml with the appropriate system to detect α-tubulin in cells and tissues.

Immunohistochemistry(Paraffin-embedded S ... :

At 1-2μg/ml to detect α-tubulin in formalin fixed and paraffin embedded tissues.

Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

Microtubules from chicken embryo brain.

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Reconstitution :

1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1001 in WB, supported by SA1021 in IHC(P).

Background :

Alpha-tubulin(b-alpha-1) mRNA is expressed only in brain with a molecular weight of about 55,000. The 3-prime UTR of b-alpha-1 is more than 80% homologous to the UTR of the rat brain alpha-tubulin gene, IL-alpha-T1. B-alpha-1 encodes a predicted 451-amino acid protein that is 100% identical to the rat homolog and differs by only 2 and 3 amino acids from the pig and chicken homologs, respectively.

Reference :

1. Cowan, N. J.; Dobner, P. R.; Fuchs, E. V.; Cleveland, D. W. : Expression of human alpha-tubulin genes: interspecies conservation of 3-prime untranslated regions. Molec. Cell. Biol. 3: 1738-1745, 1983. 2. Hall, J. L.; Cowan, N. J. : Structural features and restricted expression of a human alpha-tubulin gene. Nucleic Acids Res. 13: 207-223, 1985.

Uniprot ID :

Human(Q71U36), Mouse(P68369), Rat(P68370), Human(P68363), Mouse(P05213), Rat(Q6P9V9), Human(Q9BQE3), Mouse(P68373), Rat(Q6AYZ1), Human(Q1ZYQ1), Mouse(O89052), Mouse(P05214), Rat(Q68FR8), Human(P68366), Mouse(P68368), Rat(Q5XIF6), Human(Q9H853)

more info or order :

Boster Immunoleader product webpage