Anti-alpha-Smooth Muscle Actin Antibody (monoclonal) | Boster Immunoleader MA1106 product information

product summary

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-alpha-Smooth Muscle Actin Antibody (monoclonal)

catalog :

MA1106

quantity :

100 ug/vial

price :

200 USD

clonality :

monoclonal

host :

mouse

clone name :

1A4

The same clone is also sold as:

LifeSpan Biosciences: LS-C182754, LS-B12763, LS-C182755, LS-C202806, LS-C171306, LS-C202584, LS-C87562, LS-C87563, LS-C95300, LS-B5147, LS-C187560, LS-C86744, LS-C124868
Invitrogen: MA1-06110, MA1-37028, 180106, MA1-37027
Abnova: MAB2133
BioLegend: 904601
US Biological: 168642-AF, 168642
Abcam: ab7817, ab8211, ab8207
MyBioSource: MBS175016, MBS300924
BioLogo: AA100
eBioscience: 53-9760-82, 41-9760-80, 14-9760-82, 50-9760-80, 50-9760-82, 41-9760-82, 14-9760-80, 53-9760-80
Santa Cruz Biotechnology: sc-32251 AC, sc-32251
OriGene: TA327604

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Boster Immunoleader product webpage

citations: 23

Published Application/Species/Dilution	Reference
	Xu G, Wang X, Deng C, Teng X, Suuronen E, Shen Z, et al. Injectable biodegradable hybrid hydrogels based on thiolated collagen and oligo(acryloyl carbonate)-poly(ethylene glycol)-oligo(acryloyl carbonate) copolymer for functional cardiac regeneration. Acta Biomater. 2015;15:55-64 pubmed publisher
	Liu Z, Xu S, Huang X, Wang J, Gao S, Li H, et al. Cryptotanshinone, an orally bioactive herbal compound from Danshen, attenuates atherosclerosis in apolipoprotein E-deficient mice: role of lectin-like oxidized LDL receptor-1 (LOX-1). Br J Pharmacol. 2015;172:5661-75 pubmed publisher
	Yang L, Ni L, Duan Q, Wang X, Chen C, Chen S, et al. CYP epoxygenase 2J2 prevents cardiac fibrosis by suppression of transmission of pro-inflammation from cardiomyocytes to macrophages. Prostaglandins Other Lipid Mediat. 2015;116:64-75 pubmed publisher
	Li Q, Zhu Y, Zhang M, Chen L, Zhang Z, Du Y, et al. Chlorogenic acid inhibits hypoxia-induced pulmonary artery smooth muscle cells proliferation via c-Src and Shc/Grb2/ERK2 signaling pathway. Eur J Pharmacol. 2015;751:81-8 pubmed publisher
	Qiu X, Fu K, Zhao X, Zhang Y, Yuan Y, Zhang S, et al. Protective effects of astaxanthin against ischemia/reperfusion induced renal injury in mice. J Transl Med. 2015;13:28 pubmed publisher
	Liu T, Zeng Z, Liu Y, Wang J, Maitz M, Wang Y, et al. Surface modification with dopamine and heparin/poly-L-lysine nanoparticles provides a favorable release behavior for the healing of vascular stent lesions. ACS Appl Mater Interfaces. 2014;6:8729-43 pubmed publisher
	Gu G, Chen T, Zhou H, Sun K, Li J. Role of Wnt/β-catenin signaling pathway in the mechanism of calcification of aortic valve. J Huazhong Univ Sci Technolog Med Sci. 2014;34:33-6 pubmed publisher
	Jia Z, Tian H, Kang K, Miao H, Liu K, Jiang S, et al. Expression of the tissue inhibitor of metalloproteinase-3 by transplanted VSMCs modifies heart structure and function after myocardial infarction. Transpl Immunol. 2014;30:149-58 pubmed publisher
	Liu X, Qian L, Nan H, Cui M, Hao X, Du Y. Function of the transforming growth factor-β1/c-Jun N-terminal kinase signaling pathway in the action of thalidomide on a rat model of pulmonary fibrosis. Exp Ther Med. 2014;7:669-674 pubmed
	Tao H, Yang J, Chen Z, Xu S, Zhou X, Zhan H, et al. DNMT3A silencing RASSF1A promotes cardiac fibrosis through upregulation of ERK1/2. Toxicology. 2014;323:42-50 pubmed publisher
	You H, Wei L, Sun W, Wang L, Yang Z, Liu Y, et al. The green tea extract epigallocatechin-3-gallate inhibits irradiation-induced pulmonary fibrosis in adult rats. Int J Mol Med. 2014;34:92-102 pubmed publisher
	Tang J, Huang H, Ji X, Zhu X, Li Y, She M, et al. Involvement of IL-13 and tissue transglutaminase in liver granuloma and fibrosis after schistosoma japonicum infection. Mediators Inflamm. 2014;2014:753483 pubmed publisher
	Zhao L, Yang G, Zhao X. Rho-associated protein kinases play an important role in the differentiation of rat adipose-derived stromal cells into cardiomyocytes in vitro. PLoS ONE. 2014;9:e115191 pubmed publisher
	Yang L, Liu J, Bai X, Fan L, Han F, Jia W, et al. Acute downregulation of miR-155 at wound sites leads to a reduced fibrosis through attenuating inflammatory response. Biochem Biophys Res Commun. 2014;453:153-9 pubmed publisher
	Fan L, Hu K, Ji K, Sun Q, Xiong J, Yang L, et al. Directed differentiation of aged human bone marrow multipotent stem cells effectively generates dopamine neurons. In Vitro Cell Dev Biol Anim. 2014;50:304-12 pubmed publisher
	Zhu X, Wang Y, Yang Y, Yang R, Zhu B, Zhang Y, et al. Suppression of lipopolysaccharide-induced upregulation of toll-like receptor 4 by emodin in mouse proximal tubular epithelial cells. Mol Med Rep. 2012;6:493-500 pubmed publisher
	Li W, Man X, Li C, Chen J, Zhou J, Cai S, et al. VEGF induces proliferation of human hair follicle dermal papilla cells through VEGFR-2-mediated activation of ERK. Exp Cell Res. 2012;318:1633-40 pubmed publisher
	Zhang R, Zhou S, Li C, Wang X, Tang Y, Chen R, et al. C-reactive protein/oxidised low-density lipoprotein/β2-glycoprotein I complex promotes atherosclerosis in diabetic BALB/c mice via p38mitogen-activated protein kinase signal pathway. Lipids Health Dis. 2013;12:42 pubmed publisher
	Gong C, Hou L, Zhu Y, Lv J, Liu Y, Luo L. In vitro constitution of esophageal muscle tissue with endocyclic and exolongitudinal patterns. ACS Appl Mater Interfaces. 2013;5:6549-55 pubmed publisher
	Yu K, Zhu P, Dong Q, Zhong Y, Zhu Z, Lin Y, et al. Thymic stromal lymphopoietin attenuates the development of atherosclerosis in ApoE-/- mice. J Am Heart Assoc. 2013;2:e000391 pubmed publisher
	Hu J, Zhou Z, Shi S, Zhu X, Wang X, Zhang W, et al. Mesenchymal stem-like cells isolated from human esophageal carcinoma and adjacent non-cancerous tissues. Oncol Lett. 2013;5:179-184 pubmed
	Su Y, Liu W, Ma L, Liu X, Liu Z, Zhu B. Scutellarin inhibits translocation of protein kinase C in diabetic thoracic aorta of the rat. Clin Exp Pharmacol Physiol. 2012;39:136-40 pubmed publisher
	Zhang S, Zheng Y, Yu P, Min T, Yu F, Ye C, et al. Lentiviral vector-mediated down-regulation of IL-17A receptor in hepatic stellate cells results in decreased secretion of IL-6. World J Gastroenterol. 2012;18:3696-704 pubmed publisher

product information

Product Name :

Anti-alpha-Smooth Muscle Actin Antibody (monoclonal)

Catalog Number :

MA1106

Clonality :

Monoclonal (Clone: 1A4)

Description short :

Mouse IgG monoclonal antibody for alpha-Smooth Muscle Actin, actin, alpha 2, smooth muscle, aorta (ACTA2 ) detection. Tested with WB, IHC-P, IHC-F in Human;mouse;rat. No cross reactivity with other proteins.

Price :

200 USD

Ig type :

Mouse IgG2a

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Human, mouse, rat.

Applications :

WB,IHC-P,IHC-F

Western blot :

At 2μg/ml with the appropriate system to detect α-SMA in cells and tissues.

Immunohistochemistry(Paraffin-embedded S ... :

At 0.1-0.5μg/ml to detect α-SMA in formalin fixed and paraffin embedded tissues.

Immunohistochemistry(Frozen Section) :

At 0.1-0.5μg/ml to detect α-SMA in formalin or acetone fixed tissues.

Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

N-terminal synthetic decapeptide of alpha-smooth muscle actin.

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Reconstitution :

1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1001 in WB, supported by SA1021 in IHC(P) and IHC(F).

Background :

Ueyama et al.(1990) assigned the ACTSA gene to chromosome 10 by Southern blot analysis of DNAs from 18 rodent-human somatic cell hybrids. Regional mapping by in situ hybridization localized the gene to 10q22-q24.Assignment of the vascular smooth muscle actin gene ACTSA to human chromosome. Smooth muscle alpha-actin gene requires two E-boxes for proper expression in vivo and is a target of class I basic helix-loop-helix proteins.

Reference :

1. Kumar, M. S.; Hendrix, J. A.; Johnson, A. D.; Owens, G. K. : Smooth muscle alpha-actin gene requires two E-boxes for proper expression in vivo and is a target of class I basic helix-loop-helix proteins. Circ. Res. 92: 840-847, 2003. 2. Ueyama, H.; Bruns, G.; Kanda, N. : Assignment of the vascular smooth muscle actin gene ACTSA to human chromosome 10. Jpn. J. Hum. Genet. 35: 145-150, 1990.

Gene Name :

ACTA2

Protein Name :

Actin, aortic smooth muscle

Gene Full Name :

actin, alpha 2, smooth muscle, aorta

Synonyms :

AAT6 ACTSA MYMY5 ACTSA alpha-cardiac actin cell growth-inhibiting gene 46 protein

Uniprot ID :

Human(P62736), Mouse(P62737), Rat(P62738)

more info or order :

Boster Immunoleader product webpage