Anti-alpha-Actinin Antibody (monoclonal) | Boster Immunoleader MA1104 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-alpha-Actinin Antibody (monoclonal)

catalog :

MA1104

quantity :

100 ug/vial

price :

200 USD

clonality :

monoclonal

host :

mouse

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

publications citing this reagent :

Hao L, Wang X, Cheng J, You S, Ma S, Zhong X, et al. The up-regulation of endothelin-1 and down-regulation of miRNA-125a-5p, -155, and -199a/b-3p in human atherosclerotic coronary artery. Cardiovasc Pathol. 2014;23:217-23 pubmed publisher
Leng X, Zhan R, Wang Y, Liu X, Gong J, Gao X, et al. Anti-heat shock protein 70 autoantibody epitope changes and BD091 promotes atherosclerosis in rats. Cell Stress Chaperones. 2010;15:947-58 pubmed publisher

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-alpha-Actinin Antibody (monoclonal)

Catalog Number :

MA1104

Clonality :

Monoclonal (Clone: SA-20)

Description short :

Mouse IgG monoclonal antibody for alpha-Actinin, actinin, alpha 2 (ACTN2 ) detection. Tested with WB, IHC-P, IHC-F in Human;mouse;rat. No cross reactivity with other proteins.

Price :

200 USD

Ig type :

Mouse IgG1

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Human, mouse, rat, rabbit.

Applications :

WB,IHC-P,IHC-F

Western blot :

At 3.5μg/ml with the appropriate system to detect α-actinin in cells and tissues.

Immunohistochemistry(Paraffin-embedded S ... :

At 3.5-7μg/ml to detect α-actinin in formalin fixed and paraffin embedded tissues. Boiling the sections is required.

Immunohistochemistry(Frozen Section) :

At 3.5-7μg/ml to detect α-actinin in formalin or acetone fixed tissues.

Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

Rabbit skeletal alpha-actinin.

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Reconstitution :

1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1001 in WB, supported by SA1021 in IHC(P) and IHC(F).

Background :

Alpha-actinin was initially isolated from rabbit skeletal muscle as a factor that induces the gelation of F-actin and promotes the superprecipitation of actomyosin. Alpha actinins are actin-binding proteins that carry out different purposes in different different cell types. In myofibrillar cells, alpha-actinin constitutes a major component of Z-discs in striated muscle and of the functionally analogous dense bodies and dense plaques in smooth muscle. Alpha-actinin(alpha A) shares structural homology with spectrin and dystrophin.

Reference :

1. Youssoufian, H.; McAfee, M.; Kwiatkowski, D. J. : Cloning and chromosomal localization of the human cytoskeletal alpha-actinin gene reveals linkage to the beta-spectrin gene. Am. J. Hum. Genet. 47: 62-72, 1990.

Gene Name :

ACTN2

Protein Name :

Alpha-actinin-2

Gene Full Name :

actinin, alpha 2

Synonyms :

CMD1AA Alpha-actinin skeletal muscle isoform 2 F-actin cross-linking protein

Uniprot ID :

Human(P35609), Mouse(Q9JI91), Rat(D3ZCV0)

more info or order :

Boster Immunoleader product webpage