Anti-Vinculin Antibody (monoclonal) | Boster Immunoleader MA1103 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Vinculin Antibody (monoclonal)

catalog :

MA1103

quantity :

100 ug/vial

price :

200 USD

clonality :

monoclonal

host :

mouse

reactivity :

human, mouse, rat, chicken

application :

western blot, immunohistochemistry - frozen section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Vinculin Antibody (monoclonal)

Catalog Number :

MA1103

Clonality :

Monoclonal (Clone: VIN-54)

Description short :

Mouse IgG monoclonal antibody for Vinculin, vinculin (VCL) detection. Tested with WB, IHC-F in Human;mouse;rat;chicken. No cross reactivity with other proteins.

Price :

200 USD

Ig type :

Mouse IgG1

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Human, chicken, mouse, rat

Applications :

WB,IHC-F

Western blot :

At 1-2μg/ml with the appropriate system to detect vinculin in cells and tissues.

Immunohistochemistry(Frozen Section) :

At 2-4μg/ml to detect vinculin in formalin or acetone fixed tissues.

Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

Human vinculin, purified from uterus.

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Reconstitution :

1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1001 in WB, supported by SA1021 in IHC(F).

Background :

Vinculin is a cytoskeletal protein associated with the cytoplasmic face of both cell-cell and cell-extracellular matrix adherens-type junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Both human and chicken embryo sequences of vinculin contain 1,066 amino acids and, furthermore, that the 2 proteins exhibit a high level of sequence identity(greater than 95%). Vinculinis mapped to 10q22.1-q23.

Reference :

1. Weller, P. A.; Ogryzko, E. P.; Corben, E. B.; Zhidkova, N. I.; Patel, B.; Price, G. J.; Spurr, N. K.; Koteliansky, V. E.; Critchley, D. R. : Complete sequence of human vinculin and assignment of the gene to chromosome 10. Proc. Nat. Acad. Sci. 87: 5667-5671, 1990. 2. Mulligan, L. M.; Gardner, E.; Telenius, H.; Ponder, B. A. J. : Complementary physical and genetic techniques map the vinculin (VCL) gene on chromosome 10q. Genomics 13: 1347-1349, 1992.

Gene Name :

VCL

Protein Name :

Vinculin

Gene Full Name :

vinculin

Synonyms :

MVCL CMD1W CMH15 metavinculin

Uniprot ID :

Human(P18206), Mouse(Q64727), Rat(P85972)

more info or order :

Boster Immunoleader product webpage