Anti-Vimentin Antibody (monoclonal) | Boster Immunoleader MA1102 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Vimentin Antibody (monoclonal)

catalog :

MA1102

quantity :

100 ug/vial

price :

200 USD

clonality :

monoclonal

host :

mouse

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

publications citing this reagent :

Yang D, Lu X, Hong Y, Xi T, Zhang D. The molecular mechanism for effects of TiN coating on NiTi alloy on endothelial cell function. Biomaterials. 2014;35:6195-205 pubmed publisher
Huang F, Cao J, Liu Q, Zou Y, Li H, Yin T. MAPK/ERK signal pathway involved expression of COX-2 and VEGF by IL-1β induced in human endometriosis stromal cells in vitro. Int J Clin Exp Pathol. 2013;6:2129-36 pubmed
Luo J, Liang M, Yang X, Xu H, Shi D, Lu S. Establishment and biological characteristics comparison of Chinese swamp buffalo (Bubalus bubalis) fibroblast cell lines. In Vitro Cell Dev Biol Anim. 2014;50:7-15 pubmed publisher
Zhu X, Wang Y, Yang Y, Yang R, Zhu B, Zhang Y, et al. Suppression of lipopolysaccharide-induced upregulation of toll-like receptor 4 by emodin in mouse proximal tubular epithelial cells. Mol Med Rep. 2012;6:493-500 pubmed publisher
Chen B, Sun H, Wang H, Kong H, Chen F, Yu Q. The effects of human platelet lysate on dental pulp stem cells derived from impacted human third molars. Biomaterials. 2012;33:5023-35 pubmed publisher

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Vimentin Antibody (monoclonal)

Catalog Number :

MA1102

Clonality :

Monoclonal (Clone: VMT-24)

Description short :

Mouse IgG monoclonal antibody for Vimentin, vimentin (VIM) detection. Tested with WB, IHC-P in Human;rat. No cross reactivity with other proteins.

Price :

200 USD

Ig type :

Mouse IgG1

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Human, rabbit, pig, rat.

Applications :

WB,IHC-P

Western blot :

At 0.5-1μg/ml with the appropriate system to detect vimentin in cells and tissues.

Immunohistochemistry(Paraffin-embedded S ... :

At 1.5-2.5μg/ml to detect vimentin in formalin fixed and paraffin embedded tissues.

Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

Pig eye lens vimentin.

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Reconstitution :

1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1001 in WB, supported by SA1021 in IHC(P).

Background :

The VIM gene was one of many that Gieser and Swaroop(1992) recovered from a subtracted cDNA library for retinal pigment epithelium. Vimentin gene express in human lymphocytes and in Burkitt's lymphoma cells. Vimentin is secreted by activated macrophages. The gene encoding human vimentin is located on the short arm of chromosome 10.

Reference :

1. Lilienbaum, A.; Legagneux, V.; Portier, M.-M.; Dellagi, K.; Paulin, D. : Vimentin gene: expression in human lymphocytes and in Burkitt's lymphoma cells. EMBO J. 5: 2809-2814, 1986. 2. Mor-Vaknin, N.; Punturieri, A.; Sitwala, K.; Markovitz, D. M. : Vimentin is secreted by activated macrophages. Nature Cell Biol. 5: 59-63, 2003 3. Ferrari, S.; Cannizzaro, L. A.; Battini, R.; Huebner, K.; Baserga, R. : The gene encoding human vimentin is located on the short arm of chromosome 10. Am. J. Hum. Genet. 41: 616-626, 1987.

Gene Name :

VIM

Protein Name :

Vimentin

Gene Full Name :

vimentin

Uniprot ID :

Human(P08670), Mouse(P20152), Rat(P31000)

more info or order :

Boster Immunoleader product webpage