Anti-Troponin T Antibody (monoclonal) | Boster Immunoleader MA1098 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Troponin T Antibody (monoclonal)

catalog :

MA1098

quantity :

100 ug/vial

price :

200 USD

clonality :

monoclonal

host :

mouse

reactivity :

human, mouse, rat, chicken

application :

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

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product information

Product Name :

Anti-Troponin T Antibody (monoclonal)

Catalog Number :

MA1098

Clonality :

Monoclonal (Clone: TT-98)

Description short :

Mouse IgG monoclonal antibody for Troponin T, troponin T type 1 (skeletal, slow); troponin T type 2 (cardiac); troponin T type 3 (skeletal, fast) (TNNT1; TNNT2; TNNT3) detection. Tested with WB, IHC-P, IHC-F in Human;mouse;rat;rabbit;chicken. No cross reactivity with other proteins.

Price :

200 USD

Ig type :

Mouse IgG1

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Rat, rabbit, chicken.

Applications :

WB,IHC-P,IHC-F

Western blot :

At 2-4μg/ml with the appropriate system to detect Troponin T in cells and tissues.

Immunohistochemistry(Paraffin-embedded S ... :

At 4-8μg/ml to detect Troponin T in formalin fixed and paraffin embedded tissues.

Immunohistochemistry(Frozen Section) :

At 4-8μg/ml to detect Troponin T in formalin or acetone fixed tissues.

Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

Troponin T from rabbit skeletal muscle.

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Reconstitution :

1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1001 in WB, supported by SA1021 in IHC(P) and IHC(F).

Background :

The troponin complex is located on the thin filament of striated muscle and is composed of 3 component polypeptides: troponin 1, troponin T and troponin C. Three troponin T genes have been described on the basis of molecular cloning in humans and other vertebrates. These are expressed in a tissue-specific manner and encode the troponin T isoforms expressed in slow skeletal muscle(TNNT1), cardiac muscle(TNNT2),and fast skeletal muscle(TNNT3). TNNT1, TNNT2, TNNT3 are located on 19q13.4, 1q32, 11p15.5 respectively.

Reference :

1. Samson, F.; de Jong, P. J.; Trask, B. J.; Koza-Taylor, P.; Speer, M. C.; Potter, T.; Roses, A. D.; Gilbert, J. R. : Assignment of the human slow skeletal troponin T gene to 19q13.4 using somatic cell hybrids and fluorescence in situ hybridization analysis. Genomics 13: 1374-1375, 1992. 2. Durand, J.-B.; Bachinski, L. L.; Bieling, L. C.; Czernuszewicz, G. Z.; Abchee, A. B.; Yu, Q. T.; Tapscott, T.; Hill, R.; Ifegwu, J.; Marian, A. J.; Brugada, R.; Daiger, S.; Gregoritch, J. M.; Anderson, J. L.; Quinones, M.; Towbin, J. A.; Roberts, R. : Localization of a gene responsible for familial dilated cardiomyopathy to chromosome 1q32. Circulation 92: 3387-3389, 1995. 3. Mao, C.; Baumgartner, A. P.; Jha, P. K.; Huang, T. H.-M.; Sarkar, S. : Assignment of the human fast skeletal troponin T gene (TNNT3) to chromosome 11p15.5: evidence for the presence of 11pter in a monochromosome 9 somatic cell hybrid in NIGMS mapping panel 2. Genomics 31: 385-388, 1996.

Gene Name :

TNNT1; TNNT2; TNNT3

Protein Name :

Troponin T, slow skeletal muscle; Troponin T, cardiac muscle; Troponin T, fast skeletal muscle

Gene Full Name :

troponin T type 1(skeletal, slow); troponin T type 2(cardiac); troponin T type 3(skeletal, fast)

Synonyms :

TNT TNTF

Uniprot ID :

Human(P13805), Mouse(O88346), Rat(Q7TNB2), Human(P45379), Mouse(P50752), Rat(P50753), Human(P45378), Mouse(Q9QZ47), Rat(P09739)

more info or order :

Boster Immunoleader product webpage