Anti-Tropomyosin(Sarcomeric) Antibody (monoclonal) | Boster Immunoleader MA1097 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Tropomyosin(Sarcomeric) Antibody (monoclonal)

catalog :

MA1097

quantity :

100 ug/vial

price :

200 USD

clonality :

monoclonal

host :

mouse

reactivity :

human, mouse, rat, chicken

application :

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Tropomyosin(Sarcomeric) Antibody (monoclonal)

Catalog Number :

MA1097

Clonality :

Monoclonal (Clone: ST-39)

Description short :

Mouse IgG monoclonal antibody for Tropomyosin (Sarcomeric), tropomyosin 1 (alpha) (TPM1) detection. Tested with WB, IHC-P, IHC-F in Human;rat;chicken. No cross reactivity with other proteins.

Price :

200 USD

Ig type :

Mouse IgG1

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Human, rat, chicken.

Applications :

WB,IHC-P,IHC-F

Western blot :

At 1-2μg/ml with the appropriate system to detect tropomyosin in cells and tissues.

Immunohistochemistry(Paraffin-embedded S ... :

At 2-4μg/ml to detect tropomyosin in formalin fixed and paraffin embedded tissues.

Immunohistochemistry(Frozen Section) :

At 2-4μg/ml to detect tropomyosin in formalin or acetone fixed tissues.

Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

Chicken muscle tropomyosin.

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Reconstitution :

1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1001 in WB, supported by SA1021 in IHC(P) and IHC(F).

Background :

Tropomyosin is an alpha-helical, parallel, two-chain coiled coil which binds along the length of actin filaments in both muscle and non-muscle cells. This gene associates N-terminus to C-terminus to form a continuous strand along both sides of the actin filament and regulates its function. Tropomyosin contributes to most, if not all, functions of the actin cytoskeleton, and its role is essential for the viability of a wide range of organisms. The ability of tropomyosin to contribute to the many functions of the actin cytoskeleton is related to the temporal and spatial regulation of expression of tropomyosin isoforms.

Reference :

1. Coulton AT, Koka K, Lehrer SS, Geeves MA. Role of the Head-to-Tail Overlap Region in Smooth and Skeletal Muscle beta-Tropomyosin.Biochemistry. 2008 Jan 8;47(1):388-97. Epub 2007 Dec 11. 2. Singh A, Hitchcock-Degregori SE. Tropomyosin's Periods Are Quasi-Equivalent for Actin Binding but Have Specific Regulatory Functions. Biochemistry. 2007 Dec 25;46(51):14917-14927. Epub 2007 Dec. 3. Gunning P, O'neill G, Hardeman E. Tropomyosin-based regulation of the actin cytoskeleton in time and space.Physiol Rev. 2008 Jan;88(1):1-35.

Gene Name :

TPM1

Protein Name :

Tropomyosin alpha-1 chain

Gene Full Name :

tropomyosin 1(alpha)

Synonyms :

CMH3 TMSA CMD1Y C15orf13 HTM-alpha Alpha-tropomyosin Tropomyosin-1

Uniprot ID :

Human(P09493), Mouse(P58771), Rat(P04692)

more info or order :

Boster Immunoleader product webpage