Anti-Tropomyosin(36kD) Antibody (monoclonal) | Boster Immunoleader MA1096 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Tropomyosin(36kD) Antibody (monoclonal)

catalog :

MA1096

quantity :

100 ug/vial

price :

200 USD

clonality :

monoclonal

host :

mouse

application :

western blot, immunohistochemistry - frozen section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Tropomyosin(36kD) Antibody (monoclonal)

Catalog Number :

MA1096

Clonality :

Monoclonal (Clone: TM-36)

Description short :

Mouse IgG monoclonal antibody for Tropomyosin (36kD) detection. Tested with WB, IHC-F in Human;rabbit;chicken. No cross reactivity with other proteins.

Price :

200 USD

Ig type :

Mouse IgG2a

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Human, chicken, rabbit

Applications :

WB,IHC-F

Western blot :

At 0.5-1μg/ml with the appropriate system to detect tropomyosin in cells and tissues.

Immunohistochemistry(Frozen Section) :

At 1-2μg/ml to detect tropomyosin in formalin or acetone fixed tissues.

Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

Chicken gizzard tropomyosin.

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Reconstitution :

1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1001 in WB, supported by SA1021 in IHC(F).

Background :

The tropomyosins are a family of actin filament binding proteins. These proteins were first isolated from skeletal muscle, but later identified in many nonmuscle tissues. Tropomyosins are ubiquitous proteins of 35 to 45 kD associated with the actin filaments of myofibrils and stress fibers. Vertebrates have at least 4 different tropomyosin genes; in humans, they are named TPM1, TPM2, TPM3, and TPM4. Tropomyosins expressed as different isoforms in muscle and non-muscle cells.

Reference :

1. Lees-Miller, J. P.; Helfman, D. M. : The molecular basis for tropomyosin isoform diversity. BioEssays 13: 429-437, 1991. 2. Gariboldi, M.; Manenti, G.; Dragani, T. A.; Pierotti, M. A. : Chromosome mapping of nine tropomyosin-related sequences in mice. Mammalian Genome 6: 273-277, 1995.

Uniprot ID :

Human(P09493), Mouse(P58771), Rat(P04692), Human(P06753), Mouse(P21107), Rat(Q63610), Human(P67936), Mouse(Q6IRU2), Rat(P09495), Human(P07951), Mouse(P58774), Rat(P58775)

more info or order :

Boster Immunoleader product webpage