Anti-Synaptophysin Antibody (monoclonal) | Boster Immunoleader MA1091 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Synaptophysin Antibody (monoclonal)

catalog :

MA1091

quantity :

100 ug/vial

price :

200 USD

clonality :

monoclonal

host :

mouse

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Synaptophysin Antibody (monoclonal)

Catalog Number :

MA1091

Clonality :

Monoclonal (Clone: Syn-58)

Description short :

Mouse IgG monoclonal antibody for Synaptophysin, synaptophysin (SYP) detection. Tested with WB, IHC-F in Human;Rat. No cross reactivity with other proteins.

Price :

200 USD

Ig type :

Mouse IgG1

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Human, rat

Applications :

WB,IHC-P,IHC-F

Western blot :

At 0.5-1μg/ml with the appropriate system to detect synaptophysin in cells and tissues.

Immunohistochemistry(Paraffin-embedded S ... :

At 1-2μg/ml to detect synaptophysin in formalin fixed and paraffin embedded tissues.

Immunohistochemistry(Frozen Section) :

At 1-2μg/ml to detect synaptophysin in formalin or acetone fixed tissues.

Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

Rat retina synaptosome.

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Reconstitution :

1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1001 in WB, supported by SA1021 in IHC(P) and IHC(F).

Background :

Synaptophysin(SYP) is an integral membrane protein of small synaptic vesicles in brain and endocrine cells. Ozcelik et al.(1990) concluded that the gene has 7 exons distributed over about 20 kb. SYP mapped the SYP locus to Xp11.23-p11.22. Regionalization of the gene on the X chromosome was also done with hamster/human hybrid cells in which various portions of the human X chromosome were present.

Reference :

1. McMahon, H. T.; Bolshakov, V. Y.; Janz, R.; Hammer, R. E.; Siegelbaum, S. A.; Sudhof, T. C. : Synaptophysin, a major synaptic vesicle protein, is not essential for neurotransmitter release. Proc. Nat. Acad. Sci. 93: 4760-4764, 1996. 2. Cremin, S. M.; Greer, W. L.; Bodok-Nutzati, R.; Schwartz, M.; Peacocke, M.; Siminovitch, K. A. : Linkage of Wiskott-Aldrich syndrome with three marker loci, DXS426, SYP and TFE3, which map to the Xp11.3-p11.22 region. Hum. Genet. 92: 250-253, 1993. 3. Ozcelik, T.; Lafreniere, R. G.; Archer, B. T., III; Johnston, P. A.; Willard, H. F.; Francke, U.; Sudhof, T. C. : Synaptophysin: structure of the human gene and assignment to the X chromosome in man and mouse. Am. J. Hum. Genet. 47: 551-561, 1990.

Gene Name :

SYP

Protein Name :

Synaptophysin

Gene Full Name :

synaptophysin

Synonyms :

MRXSYP Major synaptic vesicle protein p38

Uniprot ID :

Human(P08247), Mouse(Q62277), Rat(P07825)

more info or order :

Boster Immunoleader product webpage