Anti-Spectrin(alpha and beta) Antibody (monoclonal) | Boster Immunoleader MA1090 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Spectrin(alpha and beta) Antibody (monoclonal)

catalog :

MA1090

quantity :

100 ug/vial

price :

200 USD

clonality :

monoclonal

host :

mouse

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Spectrin(alpha and beta) Antibody (monoclonal)

Catalog Number :

MA1090

Clonality :

Monoclonal (Clone: Spe 1/2)

Description short :

Mouse IgG monoclonal antibody for Spectrin(alpha and beta), spectrin, alpha, erythrocytic 1 (elliptocytosis 2); spectrin, beta, erythrocytic (SPTA1; SPTB ) detection. Tested with WB in Human. No cross reactivity with other proteins.

Price :

200 USD

Ig type :

Mouse IgG1

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Human

Applications :

Western blot :

At 2-4μg/ml with the appropriate system to detect spectrin in cells and tissues.

Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

Human erythrocyte spectrin.

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Reconstitution :

1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1001 in WB.

Background :

Spectrin, the predominant component of the membrane skeleton of the red cell, is essential in determining the properties of the membrane including its shape and deformability. It consists of 2 nonidentical subunits, alpha and beta. Spectrin is present in the red cell membrane in a tetrameric or possibly higher polymeric form through head-to-head self-association of heterodimers that are linked by actin polymers and protein 4.1 to form a 2-dimensional network. Non-erythroid spectrin gene is mapped to human chromosome 2. Spectrin mutations cause spinocerebellar ataxia type 5.

Reference :

1. Watkins, P. C.; Eddy, R.; Forget, B. G.; Chang, J. G.; Rochelle, R.; Shows, T. B. : Assignment of a non-erythroid spectrin gene to human chromosome 2. (Abstract) Am. J. Hum. Genet. 43: A161, 1988. 2. Ikeda, Y.; Dick, K. A.; Weatherspoon, M. R.; Gincel, D.; Armbrust, K. R.; Dalton, J. C.; Stevanin, G.; Durr, A.; Zuhlke, C.; Burk, K.; Clark, H. B.; Brice, A.; Rothstein, J. D.; Schut, L. J.; Day, J. W.; Ranum, L. P. W. : Spectrin mutations cause spinocerebellar ataxia type 5. Nature Genet. 38: 184-190, 2006.

Gene Name :

SPTA1; SPTB

Protein Name :

Spectrin alpha chain, erythrocytic 1; Spectrin beta chain, erythrocytic

Gene Full Name :

spectrin, alpha, erythrocytic 1(elliptocytosis 2); spectrin, beta, erythrocytic

Synonyms :

EL2 HPP HS3 SPH3 SPTA EL3 HS2 SPH2 HSPTB1 Erythroid alpha-spectrin

Uniprot ID :

Human(P02549), Mouse(P08032), Rat(D4A678), Human(P11277), Mouse(P15508), Rat(Q6XDA0)

more info or order :

Boster Immunoleader product webpage