Anti-Smad4(DPC4) Antibody (monoclonal) | Boster Immunoleader MA1089 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Smad4(DPC4) Antibody (monoclonal)

catalog :

MA1089

quantity :

100 ug/vial

price :

200 USD

clonality :

monoclonal

host :

mouse

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Smad4(DPC4) Antibody (monoclonal)

Catalog Number :

MA1089

Clonality :

Monoclonal (Clone: IMD-89)

Description short :

Mouse IgG monoclonal antibody for Smad4 (DPC4), SMAD family member 4 (SMAD4) detection. Tested with WB, ICC in Human. No cross reactivity with other proteins.

Price :

200 USD

Ig type :

Mouse IgG1

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Human

Applications :

WB,ICC

Western blot :

At 2-4μg/ml with the appropriate system to detect Smad4 in cells and tissues.

Immunocytochemistry :

Suitable

Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

Recombinant human Smad4(DPC4).

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Reconstitution :

1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1001 in WB, supported by SA1021 in ICC.

Background :

SMAD4 plays a pivotal role in signal transduction of the transforming growth factor beta superfamily cytokines by mediating transcriptional activation of target genes. Smad4 signalling in T cells is required for suppression of gastrointestinal cancer. Mutational inactivation of SMAD4 causes TGF-beta unresponsiveness and gave a basis for understanding the physiologic role of this gene in tumorigenesis. Mutations in DPC4(SMAD4) cause juvenile polyposis syndrome, but only account for a minority of cases.

Reference :

1. Kim, B.-G.; Li, C.; Qiao, W.; Mamura, M.; Kasprzak, B.; Anver, M.; Wolfraim, L.; Hong, S.; Mushinski, E.; Potter, M.; Kim, S.-J.; Fu, X.-Y.; Deng, C.; Letterio, J. J. : Smad4 signalling in T cells is required for suppression of gastrointestinal cancer. Nature 441: 1015-1019, 2006. Note: Erratum: Nature 444: 780 only, 2006. 2. Houlston, R.; Bevan, S.; Williams, A.; Young, J.; Dunlop, M.; Rozen, P.; Eng, C.; Markie, D.; Woodford-Richens, K.; Rodriguez-Bigas, M. A.; Leggett, B.; Neale, K.; Phillips, R.; Sheridan, E.; Hodgson, S.; Iwama, T.; Eccles, D.; Bodmer, W.; Tomlinson, I. : Mutations in DPC4 (SMAD4) cause juvenile polyposis syndrome, but only account for a minority of cases. Hum. Molec. Genet. 7: 1907-1912, 1998. 3. Howe, J. R.; Roth, S.; Ringold, J. C.; Summers, R. W.; Jarvinen, H. J.; Sistonen, P.; Tomlinson, I. P. M.; Houlston, R. S.; Bevan, S.; Mitros, F. A.; Stone, E. M.; Aaltonen, L. A. : Mutations in the SMAD4/DPC4 gene in juvenile polyposis. Science 280: 1086-1088, 1998.

Gene Name :

SMAD4

Protein Name :

Mothers against decapentaplegic homolog 4

Gene Full Name :

SMAD family member 4

Synonyms :

JIP DPC4 MADH4 MYHRS MAD homolog 4 MAD,mothers against decapentaplegic homolog 4(Drosophila) SMAD, mothers against DPP homolog 4(Drosophila) SMA- AND MAD-RELATED PROTEIN 4 DELETED IN PANCREATIC CARCINOMA 4 Deletion target in pancreatic carcinoma 4 SMAD family member 4

Uniprot ID :

Human(Q13485), Mouse(P97471), Rat(O70437)

more info or order :

Boster Immunoleader product webpage