Anti-PKB alpha Antibody (monoclonal) | Boster Immunoleader MA1085 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-PKB alpha Antibody (monoclonal)

catalog :

MA1085

quantity :

100 ug/vial

price :

200 USD

clonality :

monoclonal

host :

mouse

reactivity :

human, mouse, rat, chicken

application :

western blot

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-PKB alpha Antibody (monoclonal)

Catalog Number :

MA1085

Clonality :

Monoclonal (Clone: IML-26)

Description short :

Mouse IgG monoclonal antibody for PKB alpha, v-akt murine thymoma viral oncogene homolog 1 (AKT1) detection. Tested with WB in Human;mouse;rat;chicken. No cross reactivity with other proteins.

Price :

200 USD

Ig type :

Mouse IgG1

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Human, mouse, rat, chicken

Applications :

Western blot :

At 0.25-0.5μg/ml with the appropriate system to detect PKBα in cells and tissues.

Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

Synthetic peptide corresponding to amino acids 461-477 of human PKB alpha/Akt1, conjugated to KLH.

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Reconstitution :

1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1001 in WB.

Background :

PKB alpha also knows as V-AKT murine thymoma vial oncogene homolog 1(ATK1). AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and is abrogated by mutations in the pleckstrin homology domain of AKT1. AKT1 gene is mapped to chromosome 14q32.3. Akt1/protein kinase B-alpha is critical for ischemic and VEGF-mediated angiogenesis. Akt1 regulates pathological angiogenesis, vascular maturation and permeability in vivo.

Reference :

1. Staal, S. P.; Huebner, K.; Croce, C. M.; Parsa, N. Z.; Testa, J. R. : The AKT1 proto-oncogene maps to human chromosome 14, band q32. Genomics 2: 96-98, 1988. 2. Ackah, E.; Yu, J.; Zoellner, S.; Iwakiri, Y.; Skurk, C.; Shibata, R.; Ouchi, N.; Easton, R. M.; Galasso, G.; Birnbaum, M. J.; Walsh, K.; Sessa, W. C. : Akt1/protein kinase B-alpha is critical for ischemic and VEGF-mediated angiogenesis. J. Clin. Invest. 115: 2119-2127, 2005. 3. Chen, J.; Somanath, P. R.; Razorenova, O.; Chen, W. S.; Hay, N.; Bornstein, P.; Byzova, T. V. : Akt1 regulates pathological angiogenesis, vascular maturation and permeability in vivo. Nature Med. 11: 1188-1196, 2005.

Gene Name :

AKT1

Protein Name :

RAC-alpha serine/threonine-protein kinase

Gene Full Name :

v-akt murine thymoma viral oncogene homolog 1

Synonyms :

AKT PKB RAC CWS6 PRKBA PKB-ALPHA RAC-ALPHA ONCOGENE AKT1 PROTEIN KINASE B-ALPHA RAC SERINE/THREONINE PROTEIN KINASE Protein kinase B Proto-oncogene c-Akt RAC-PK-alpha

Uniprot ID :

Human(P31749), Mouse(P31750), Rat(P47196)

more info or order :

Boster Immunoleader product webpage