Anti-Pan-Cadherin Antibody (monoclonal) | Boster Immunoleader MA1079 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Pan-Cadherin Antibody (monoclonal)

catalog :

MA1079

quantity :

100 ug/vial

price :

200 USD

clonality :

monoclonal

host :

mouse

reactivity :

human, mouse, rat, chicken

application :

western blot, immunohistochemistry - paraffin section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Pan-Cadherin Antibody (monoclonal)

Catalog Number :

MA1079

Clonality :

Monoclonal (Clone: PC-79)

Description short :

Mouse IgG monoclonal antibody for Pan-Cadherin, cadherin 1, type 1, E-cadherin (epithelial); cadherin 2, type 1, N-cadherin (neuronal); cadherin 3, type 1, P-cadherin (placental) (CDH1; CDH2; CDH3 ) detection. Tested with WB, IHC-P in Human;mouse;rat;rabbit;chicken;snake. No cross reactivity with other proteins.

Price :

200 USD

Ig type :

Mouse IgG1

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Human, mouse, rat, rabbit, chicken, snake.

Applications :

WB,IHC-P

Western blot :

At 1-2μg/ml with the appropriate system to detect pan-cadherin in cells and tissues.

Immunohistochemistry(Paraffin-embedded S ... :

At 2-4μg/ml to detect pan-cadherin in formalin fixed and paraffin embedded tissues.

Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

Synthetic peptide corresponding to the C-terminal amino acids of chicken N-Cadherin with an extra N-terminal lysine residue(24 amino acids) coupled to KLH.

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Reconstitution :

1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1001 in WB, supported by SA1021 in IHC(P).

Background :

Cadherins are calcium-dependent cell-cell adhesion molecules that mediate cell-cell binding in a homophilic manner. They play an important role in the growth and development of cells via the mechanisms of control of tissue architecture and the maintenance of tissue integrity. Cadherin expression is regulated spatially as well as temporally. Cadherins are though to play an important role in development and maintenance of tissues through selective cell-cell adhesion activity and may be involved also in the invasion and metastasis of malignant tumors. Cadherin regulates dendritic spine morphogenesis. A cadherin gene cluster is mapped to a region of chromosome 5 subject to frequent allelic loss in carcinoma.

Reference :

1. Togashi, H.; Abe, K.; Mizoguchi, A.; Takaoka, K.; Chisaka, O.; Takeichi, M. : Cadherin regulates dendritic spine morphogenesis. Neuron 35: 77-89, 2002. 2. Chalmers, I. J.; Hofler, H.; Atkinson, M. J. : Mapping of a cadherin gene cluster to a region of chromosome 5 subject to frequent allelic loss in carcinoma. Genomics 57: 160-163, 1999.

Gene Name :

CDH1; CDH2; CDH3

Protein Name :

Cadherin-1; Cadherin-2; Cadherin-3

Gene Full Name :

cadherin 1, type 1, E-cadherin(epithelial); cadherin 2, type 1, N-cadherin(neuronal); cadherin 3, type 1, P-cadherin(placental)

Synonyms :

UVO CDHE ECAD LCAM Arc-1 CD324 CDHN NCAD CD325 CDw325 CDHP HJMD PCAD

Uniprot ID :

Human(P12830), Mouse(P09803), Rat(Q9R0T4), Human(P19022), Mouse(P15116), Rat(Q9Z1Y3), Human(P22223), Mouse(P10287), Rat(F1LMI3)

more info or order :

Boster Immunoleader product webpage