Anti-p53 Antibody (monoclonal) | Boster Immunoleader MA1078 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-p53 Antibody (monoclonal)

catalog :

MA1078

quantity :

100 ug/vial

price :

200 USD

clonality :

monoclonal

host :

mouse

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-p53 Antibody (monoclonal)

Catalog Number :

MA1078

Clonality :

Monoclonal (Clone: IMD-53)

Description short :

Mouse IgG monoclonal antibody for p53, tumor protein p53 (TP53) detection. Tested with WB, IHC-P, IHC-F, ICC in Human. No cross reactivity with other proteins.

Price :

200 USD

Ig type :

Mouse IgG2a

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Human

Applications :

WB,IHC-P,IHC-F,ICC

Western blot :

At 0.5-1μg/ml with the appropriate system to detect p53 in cells and tissues.

Immunohistochemistry(Paraffin-embedded S ... :

At 0.5-2μg/ml to detect p53 in formalin fixed and paraffin embedded tissues.

Immunohistochemistry(Frozen Section) :

At 0.5-2μg/ml to detect p53 in formalin or acetone fixed tissues.

Immunocytochemistry :

Suitable

Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

Recombinant human wild-type p53 protein.

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Reconstitution :

1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1001 in WB, supported by SA1021 in IHC(P), IHC(F) and ICC.

Background :

The p53 tumor antigen is found in increased amounts in a wide variety of transformed cells. The protein is also detectable in many actively proliferating, nontransformed cells, but it is undetectable or present at low levels in resting cells. This protein induces cell cycle arrest or apoptosis in response to sublethal or severe DNA damage, respectively, by differential transcription of target genes and through transcription-independent apoptotic functions. The p53 protein contains 393 amino acids. Human p53 tumour antigen is Locatedto band 17p13. p53 mutations are common in pancreatic cancer and are absent in chronic pancreatitis.

Reference :

1. Isobe, M.; Emanuel, B. S.; Givol, D.; Oren, M.; Croce, C. M. : Localization of gene for human p53 tumour antigen to band 17p13. Nature 320: 84-85, 1986. 2. Casey, G.; Yamanaka, Y.; Freiss, H.; Kobrin, M. S.; Lopez, M. E.; Buchler, M.; Beger, H. G.; Korc, M. : p53 mutations are common in pancreatic cancer and are absent in chronic pancreatitis. Cancer Lett. 69: 151-160, 1993.

Gene Name :

TP53

Protein Name :

Cellular tumor antigen p53

Gene Full Name :

tumor protein p53

Synonyms :

LFS1 TRP53 Li-Fraumeni syndrome TRANSFORMATION-RELATED PROTEIN 53 Antigen NY-CO-13 Phosphoprotein p53 Tumor suppressor p53

Uniprot ID :

Human(P04637), Mouse(P02340), Rat(P10361)

more info or order :

Boster Immunoleader product webpage