Anti-p34cdc2 Antibody (monoclonal) | Boster Immunoleader MA1077 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-p34cdc2 Antibody (monoclonal)

catalog :

MA1077

quantity :

100 ug/vial

price :

200 USD

clonality :

monoclonal

host :

mouse

reactivity :

human, mouse, rat, chicken

application :

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-p34cdc2 Antibody (monoclonal)

Catalog Number :

MA1077

Clonality :

Monoclonal (Clone: IMD-34)

Description short :

Mouse IgG monoclonal antibody for p34cdc2, cyclin-dependent kinase 1 (CDK1) detection. Tested with WB, IHC-P, IHC-F in Human;mouse;chicken. No cross reactivity with other proteins.

Price :

200 USD

Ig type :

Mouse IgG2a

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Human, chicken, mouse

Applications :

WB,IHC-P,IHC-F

Western blot :

At 0.5-1μg/ml with the appropriate system to detect p34cdc2 in cells and tissues.

Immunohistochemistry(Paraffin-embedded S ... :

At 1-2μg/ml to detect p34cdc2 in formalin fixed and paraffin embedded tissues.

Immunohistochemistry(Frozen Section) :

At 1-2μg/ml to detect p34cdc2 in formalin or acetone fixed tissues.

Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

C-terminal two-thirds of Xenopus p34cdc2 expressed in E.coli.

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Reconstitution :

1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1001 in WB, supported by SA1021 in IHC(P) and IHC(F).

Background :

P34(cdc2), also known as cell division cycle(CDC2), or cyclin-dependent kinase 1(CDK1). CDC2 is a catalytic subunit of a protein kinase complex, called the M-phase promoting factor, that induces entry into mitosis and is universal among eukaryotes. In HeLa cells CDC2 is the most abundant phosphotyrosine-containing protein and its phosphotyrosine content is subject to cell cycle regulation. CDC2 gene is located on chromosome 10.

Reference :

1. Draetta, G.; Piwnica-Worms, H.; Morrison, D.; Druker, B.; Roberts, T.; Beach, D. : Human CDC2 protein kinase is a major cell-cycle regulated tyrosine kinase substrate. Nature 336: 738-744, 1988. 2. Spurr, N. K.; Goodfellow, P. N.; Nurse, P.; Lee, M. : Assignment of the human homologue of the yeast cell cycle control gene CDC2 to chromosome 10. (Abstract) Cytogenet. Cell Genet. 46: 698, 1987. 3. Nazarenko, S. A.; Ostroverhova, N. V.; Spurr, N. K. : Regional assignment of the human cell cycle control gene CDC2 to chromosome 10q21 by in situ hybridization. Hum. Genet. 87: 621-622, 1991.

Gene Name :

CDK1

Protein Name :

Cyclin-dependent kinase 1

Gene Full Name :

cyclin-dependent kinase 1

Synonyms :

CDC2 CDC28A CDKN1 P34CDC2 Cell division control protein 2 homolog Cell division protein kinase 1 p34 protein kinase

Uniprot ID :

Human(P06493), Mouse(P11440), Rat(P39951)

more info or order :

Boster Immunoleader product webpage