Anti-p27Kip1 Antibody (monoclonal) | Boster Immunoleader MA1076 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-p27Kip1 Antibody (monoclonal)

catalog :

MA1076

quantity :

100 ug/vial

price :

200 USD

clonality :

monoclonal

host :

mouse

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-p27Kip1 Antibody (monoclonal)

Catalog Number :

MA1076

Clonality :

Monoclonal (Clone: IMD-27)

Description short :

Mouse IgG monoclonal antibody for p27Kip1, cyclin-dependent kinase inhibitor 1B (p27, Kip1) (CDKN1B) detection. Tested with WB, ICC in Human;mouse;rat. No cross reactivity with other proteins.

Price :

200 USD

Ig type :

Mouse IgG1

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Human, mouse, rat

Applications :

WB,ICC

Western blot :

At 2-4μg/ml with the appropriate system to detect p27Kip1 in cells and tissues.

Immunocytochemistry :

Suitable

Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

recombinant rodent p27Kip1 protein.

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Reconstitution :

1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1001 in WB, supported by SA1021 in ICC.

Background :

P27(kip1), also known as cyclin dependent kinase inhibitor 1B(CDKN1B), is a major target of AFX-like forkhead proteins. CDKN1B(p27) belongs to the Cip/Kip family and functions as an important cell cycle gatekeeper. Phosphorylation leads to the ubiquitination and degradation of CDKN1B. P27(kip1) mapped to 12p13. An increase in p27 causes proliferating cells to exit from the cell cycle, and a decrease in p27 is necessary for quiescent cells to resume division. Abnormally low amounts of p27 are associated with pathological states of excessive cell proliferation, especially cancers. Overexpression of p27Kip1 lengthens the G1 phase in a mouse model that targets inducible gene expression to central nervous system progenitor cells.

Reference :

1. Chang, B.; Zheng, S. L.; Isaacs, S. D.; Wiley, K. E.; Turner, A.; Li, G.; Walsh, P. C.; Meyers, D. A.; Isaacs, W. B.; Xu, J. : A polymorphism in the CDKN1B gene is associated with increased risk of hereditary prostate cancer. Cancer Res. 64: 1997-1999, 2004. 2. Malek, N. P.; Sundberg, H.; McGrew, S.; Nakayama, K.; Kyriakidis, T. R.; Roberts, J. M. : A mouse knock-in model exposes sequential proteolytic pathways that regulate p27(Kip1) in G1 and S phase. Nature 413: 323-327, 2001. 3. Mitsuhashi, T.; Aoki, Y.; Eksioglu, Y. Z.; Takahashi, T.; Bhide, P. G.; Reeves, S. A.; Caviness, V. S., Jr. : Overexpression of p27(Kip1) lengthens the G1 phase in a mouse model that targets inducible gene expression to central nervous system progenitor cells. Proc. Nat. Acad. Sci. 98: 6435-6440, 2001.

Gene Name :

CDKN1B

Protein Name :

Cyclin-dependent kinase inhibitor 1B

Gene Full Name :

cyclin-dependent kinase inhibitor 1B(p27, Kip1)

Synonyms :

KIP1 MEN4 CDKN4 MEN1B P27KIP1 Cyclin-dependent kinase inhibitor p27

Uniprot ID :

Human(P46527), Mouse(P46414), Rat(O08769)

more info or order :

Boster Immunoleader product webpage